FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002432958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002432958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3492725
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1411374.040884982356183Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1094903.1348016233742997No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC523601.4991160197267177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC513761.4709431747417847No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC503571.441768246855965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC478901.3711357178134551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA464771.330680199557652No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG425771.2190195334588323No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC347360.994524332720154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA337080.9650917263741062Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC261510.748727712602624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC225880.6467156732923434Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG214830.6150784845643444Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG206130.5901695667423TruSeq Adapter, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT195210.5589045802346305Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG184940.5295006048286081TruSeq Adapter, Index 13 (97% over 49bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC182720.523144536142983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG170300.48758490863151266No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA142440.4078191097209199Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC137110.39255881868741455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA132290.3787587055952015TruSeq Adapter, Index 13 (97% over 39bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC132090.3781860867946947No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT130300.3730611485301591Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA123350.3531626452125489No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC114090.32665039474908564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC112340.32163998024465135No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC108850.3116477821758083Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC97620.27949523652735325No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG92350.264406731134TruSeq Adapter, Index 2 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC90660.25956810226971777No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT89830.25719173424761466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT86780.2484592975398865Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG85650.24522400131702324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG82970.23755090939023257No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC78070.22352174877781675No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA76940.2202864525549535TruSeq Adapter, Index 13 (100% over 38bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC74410.21304282472854288Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA73520.21049467106628778Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG71510.20473985212119475No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT69180.1980688430952909No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG68750.1968377126742014No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC68610.19643687951384664No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACAC68100.19497670157255437No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC67720.19388872585159153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA67640.1936596783313888Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA67630.19363104739136347Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC66510.19042438210852558Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG64190.18378200402264708No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT63880.18289444488186157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG63710.18240771890143082No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC63610.18212140950117744Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC63220.18100480284018924Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA61760.17682468559648984No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC60830.17416200817413338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC60550.1733603418534239No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC59840.17132754511162487No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG59570.17055450973094075Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA58390.1671760588079508TruSeq Adapter, Index 13 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA57590.16488558360592373No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA57430.16442748856551834TruSeq Adapter, Index 13 (97% over 39bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC56550.16190796584328856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC55560.15907350278078006Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA54290.15543737339756206Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC53760.15391993357621914No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA53440.15300374349540832No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC52630.15068463735335588Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC52140.1492817212921143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC51940.14870910249160757Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAT51740.1481364836911008No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG51220.1466476748097832No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA51110.14633273446950448No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA50760.14533065156861763Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG48720.1394899398034486Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC48070.1376289287018016TruSeq Adapter, Index 13 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG46240.13238946667716467No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA45220.12946911079458015No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG44690.12795167097323723TruSeq Adapter, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC44600.12769399251300917No Hit
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC44500.1274076831127558Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC44320.1268923261922997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC44180.12649149303194498Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC44120.12631970739179293Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC43960.12586161235138754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA43650.12497405321060204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC43490.12451595817019662Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC43380.1242010178299179Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC43230.12377155372953783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG42760.12242589954834693No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC42210.1208511978469533Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTAGA41220.11801673478444481No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCAC41160.11784494914429278No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC40980.11732959222383668Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC40810.11684286624340594No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC40600.11624161650287385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA40270.11529679548203765Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC39300.11251959429957985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT39190.11220465395930111Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA38370.10985691687722338No Hit
ACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTAGA38220.1094274527768433No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA38210.10939882183681796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACA37000.10593447809375202No Hit
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC36440.10433114545233306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC35970.10298549127114216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA35950.10292822939109147No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC35070.10040870666886169No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC35030.10029418290876035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)