FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002432961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002432961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4717384
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1869903.963849455545701No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1656313.5110773259077486Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC983022.083824424723533No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA726791.5406632150361301Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC653361.3850049095006893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG622921.3204776206473758No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC606841.2863909319232862Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC600471.2728876852085818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC588811.248170596245716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC494421.0480808855077306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA418890.8879709601762332No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC412390.8741921369979634Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC325280.6895347082196404Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT288170.6108682269664713Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG254390.5392607428184774No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC247500.5246551902495112Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG240080.5089261336367784Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG204160.4327822369347079TruSeq Adapter, Index 2 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG201340.42680434749428925TruSeq Adapter, Index 14 (97% over 49bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC188820.4002642142339907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA171470.36348535544276234Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC168800.35782543884491913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC166520.35299225163777215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC148860.31555624897188783No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA141430.2998059941696499TruSeq Adapter, Index 14 (97% over 39bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC140550.29794055349320725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG137960.2924502224114043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT134410.28492486513711834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC134190.28445850496800773No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT131680.27913775940224494Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC130750.27716632777827716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA128530.27246032970816025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC125110.2652105488974398Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC116660.24729807876568877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC116640.24725568238667872No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC116560.24708609687063848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC114090.24185014406289587No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC112770.2390519830482318No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA106040.2247856015113461No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA103510.21942245956657336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC102600.21749342432161556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA100670.21340217374714462Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG98920.20969249058376424No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC98460.2087173738665328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT95970.20343902467978014Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA87800.18612010385417002No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC86500.18336433921851603Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG86430.1832159518919808TruSeq Adapter, Index 2 (97% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG86100.18251641163831478No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG85230.18067216915137713TruSeq Adapter, Index 2 (97% over 35bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT81450.1726592535184755Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG79620.16877998483905485Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA79150.1677836699323184No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG79100.16767767898479327No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG78940.1673385079527128Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC78640.16670256226756186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC78570.16655417494102664TruSeq Adapter, Index 14 (100% over 38bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA77490.16426477047448332Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC76820.16284449177764626Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC74950.15888043034020552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC74150.15718457517980303No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG74110.1570997824217829No Hit
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC73300.15538272907187542Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG70020.14842972291422535No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC69100.1464794894797625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC66110.1401412308177583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG65910.1397172670276577No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG65190.1381909973832955Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC62470.13242508983792714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA62260.1319799278583215Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG61970.13136518036267558No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC61260.1298601089078184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC61110.12954213606524292Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC59160.12540848911176194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC58670.12436977782601544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG58600.12422139049948022Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC58100.12316148102422869Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA57330.12152922043234132Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC56550.11987576165094893TruSeq Adapter, Index 14 (100% over 38bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT56000.11870986122817222TruSeq Adapter, Index 8 (97% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA54590.1157209165079629No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC54320.11514856539132706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC54090.11466100703271134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT53970.11440662875865097No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA53950.11436423237964093No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC53910.11427943962162079No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA52990.11232920618715796No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC52870.11207482791309759Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC52160.11056975645824041Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTAGAGGGAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC52010.11025178361566494Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT51230.10859832483427255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG50940.10798357733862667No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACAC50340.10671168596832481No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC50340.10671168596832481No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC48940.10374393943762052Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC47370.1004158236853307Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT47290.10024623816929044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)