FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002432985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002432985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12337971
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA125194010.147049300083458Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC3160082.561263922568792Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2007601.6271719231630548Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT1626941.3186446944963641TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1586541.2859002505355215No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1514491.2275032904518903Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCCGTC1463931.1865241051385191TruSeq Adapter, Index 19 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG1375581.114915896625142TruSeq Adapter, Index 19 (97% over 49bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1344601.0898064195482384No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA1338441.0848137023502487TruSeq Adapter, Index 19 (97% over 39bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1137970.9223315567851472No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG735950.5964919191332189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC588730.4771692201254161Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA583920.4732706860795831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA564720.45770897013779654TruSeq Adapter, Index 19 (100% over 38bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA546450.4429010248119403Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT527020.4271528924812678Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC493760.40019546163627717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT478530.38785145466787047TruSeq Adapter, Index 1 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC462310.37470504672121535No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC458730.3718034351029031Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC440070.35667939242197927Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC403420.32697434610601694Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC394960.32011746501916727No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG380980.30878659059905395Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT348080.2821209419279718Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG344320.2790734392227052No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT335430.27186804053924263TruSeq Adapter, Index 1 (97% over 35bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT328290.2660810274233908Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG325670.2639575016021678No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC316970.2569060990660458Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC298090.24160374505662235No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA294320.2385481372909695TruSeq Adapter, Index 19 (100% over 37bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC285490.23139136896982493Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC282050.22860322819692153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC263620.2136656019048837Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC262840.21303340719474864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA259460.21029389678416333TruSeq Adapter, Index 19 (100% over 38bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC259040.20995348424793672Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC253300.20530117958617344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC243030.19697728256939492No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC241800.1959803601418742Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA241210.1955021615790797Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA238300.1931435890066527No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC226030.18319867991260474No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA223680.18129399072181318No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG221490.17951898249720313Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC216850.17575823447793806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA211910.17175433464708256TruSeq Adapter, Index 19 (97% over 39bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG207140.16788822084279498No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT202540.16415989306507528Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC200680.16265235183321472Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC197980.16046398552890098No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAA193250.1566302919661588TruSeq Adapter, Index 19 (97% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC186550.1511999015073062No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG183690.1488818542368109No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG173550.1406633230050549Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT173030.14024185986496485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC172420.13974745118139764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC163290.1323475310486627No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC159680.12942160424919139Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC159040.12890288038446518No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC156600.12692524565019644Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA153390.12432352126617902No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG149890.12148675013095751No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA148080.12001973420102867Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG147100.11922543828316666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG147020.11916059780007589No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG144540.11715054282426178Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT142370.11539174472042445No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA137970.11182551815043171No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT137570.11150131573497782Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC135300.10966146702727701Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG131990.1069786920393961Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG130310.10561704189448978No Hit
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA128660.10427970693074251Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)