FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8541501
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5464346.397400175917559Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2632213.0816714767111777No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1850602.166598118995713Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1522071.7819701712848832Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1473801.7254578557094356No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1172701.372943701581256No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC980451.147866165443287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC965571.1304453397593701TruSeq Adapter, Index 5 (97% over 35bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC917911.07464718437661Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG769570.9009774745679945No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC724290.8479657146911299Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG670130.7845576556157986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA569040.6662060918801039TruSeq Adapter, Index 6 (100% over 40bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA534840.6261662909130374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG508300.5950944687590624TruSeq Adapter, Index 6 (100% over 49bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC506040.5924485637828761Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT495160.5797107557559262Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA474570.5556049223666895Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC460640.5392963133763023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC425060.4976408713175822TruSeq Adapter, Index 6 (100% over 50bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC407210.4767429050233677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG395830.46341971978929697No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC363660.4257565502831411Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT328700.3848269759612508Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA321880.37684243085612235TruSeq Adapter, Index 6 (100% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC319140.37363456376110005TruSeq Adapter, Index 5 (97% over 35bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA306870.35926940709835425TruSeq Adapter, Index 6 (100% over 38bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA280890.3288532074163546TruSeq Adapter, Index 6 (100% over 38bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC277290.3246384915250844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC273470.32016620966268106Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT250480.2932505656792641Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG248730.2912017454543411Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC218570.25589179232081105No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC215100.2518292745033923Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG212620.24892580355607286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA209680.24548378557820225TruSeq Adapter, Index 6 (100% over 40bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC197930.23172742121086212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC197380.2310835062830292Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC192140.2249487531524026TruSeq Adapter, Index 5 (97% over 35bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC189370.22170576342495307No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG189170.2214716125421047No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA186340.2181583775498007Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC180650.21149678493276536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC180080.21082945491664754No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC159880.18718021574896496Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT159460.18668849889498346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC158970.186114829232005Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG158340.18537725395103274Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC154780.18120936823633224Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT150890.17665513356493195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA150180.17582389793082037No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC149160.1746297284282938No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC148760.17416142666259712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA143350.16782764528154945Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA142490.1668207964853016No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC141920.1661534664691838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA138810.1625124202408921Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA138360.16198558075448333No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA134120.15702158203809846No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC121750.14253934993392847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG121380.14210617080065904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC121280.14198909535923487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC119880.14035003917929645No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC119030.13935489792719102No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG118300.13850024720479456No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC118050.13820755860123415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC112230.13139376791034738TruSeq Adapter, Index 6 (100% over 36bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA110040.12882981574315802No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC109030.1276473537847739Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA104180.12196919487570158TruSeq Adapter, Index 6 (100% over 40bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG104100.12187553452256225No Hit
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC103960.12171162890456842Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG102690.12022477079848144No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC102280.11974476148864234No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC101830.11921792200223356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC101680.11904230884009731Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG100890.11811741285284635Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC98770.11563541349465392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC97150.11373879134358234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC96910.11345781028416434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT92910.1087747926271975No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA91860.10754550049224369Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG88370.10345956758654011Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTAGAGGGAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC87200.10208978492187731Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA86720.1015278228030413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT86610.10139903981747471Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA86290.10102439840491736Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC85840.10049755891850859No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA85580.10019316277080574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)