FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195554
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3274247.804070690068582Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1294703.0858856780296477No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC870092.073838162969658Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC825581.9677496702461703No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC534491.273943798602044Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC529651.262407777375765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA413650.9859246240186634TruSeq Adapter, Index 7 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG381520.9093435574896663TruSeq Adapter, Index 7 (100% over 49bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA381040.9081994892688785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG375690.8954478955580122No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA346400.825635899335344No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA334330.7968673505334456TruSeq Adapter, Index 7 (100% over 40bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC326000.7770129999518538No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC262960.6267587069550291Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC231780.5524419421130082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC214890.5121850415940303No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG197900.4716897935290548Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC189410.45145408687386696No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC181150.43176657957447334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC178310.4249975092681443No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC178170.4246638227037478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT175930.41932483767340384Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT174720.416440832366834TruSeq Adapter, Index 7 (100% over 38bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT170530.4064540701895387Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC153910.36684070804475405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG148480.3538984362970897No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA147220.35089525721752124No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC147020.3504185621255262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT136080.3243433405933996TruSeq Adapter, Index 7 (100% over 38bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG131850.3142612393977053No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC117670.28046355737525963No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA115080.27429035593392437TruSeq Adapter, Index 7 (100% over 37bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA112670.26854618007538456TruSeq Adapter, Index 7 (100% over 35bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG100970.24065951719367693No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA100010.23837138075210093TruSeq Adapter, Index 7 (100% over 35bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC99670.2375609990957094Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA98080.23377127311434912Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC93870.22373684142785433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC93470.22278345124386434No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC93030.22173472204147535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC87130.20767221682762277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC82890.1975662808773287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG81490.1942294152333637Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT78400.1868644760620409Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA77870.18560123406825416TruSeq Adapter, Index 7 (100% over 40bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC77830.18550589504985515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC77540.1848146871664624Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA73770.1758289846823566No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG72080.17180091115499885No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC71110.16948893995882308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA68150.16243385259729703Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC66960.1595975167999268Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG65380.15583162557316627TruSeq Adapter, Index 7 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA64350.15337664584939198No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA63490.15132685695381348No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63050.1502781277514245Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT61980.14772780900925123Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA61240.14596403716886971No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC60610.14446244762908544No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG54760.13051911618823164No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA53890.12844549253805337Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA52870.12601434756887886No Hit
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA51700.12322568128070811No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC51630.12305883799850983Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC50440.12022250220113959Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC49210.11729082738537032No Hit
TCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAG49200.11726699263077058No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC48870.11648044572897882Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT48520.11564622931798756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG48500.11559855980878807No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG48280.11507419520759357No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG48270.1150503604529938Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA48240.11497885618919457Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAG47640.11354877091320956No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT47480.11316741483961355No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG47260.11264305023841906Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC46980.11197567710962604Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA46900.11178499907282805No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG46820.11159432103603005TruSeq Adapter, Index 7 (100% over 36bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC46730.1113798082446323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC46470.1107601046250388No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC46430.11066476560663979No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC46170.11004506198704629No Hit
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC46100.10987821870484804TruSeq Adapter, Index 7 (100% over 42bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC45980.10959220164965104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG45640.10878181999325953No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC45510.10847196818346279Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT45360.10811444686446652No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC44420.10587397993209002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT44300.10558796287689301Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA43300.103204487416918No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG43150.10284696609792175No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA42800.1020127496869305Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC42000.10010596931895048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)