FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236733
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2414065.697928096955838Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1502893.547285136920358No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC929042.1928216859547205No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC707701.6703908412448933Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC643521.5189061949384113Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC508351.199863196477097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC492101.1615081715085656Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA467191.1027128686183434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG433711.023689715637025No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA390910.9226684806429859No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG355250.8384998535428123TruSeq Adapter, Index 8 (100% over 49bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC344610.8133861633480325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC329780.7783827774844437TruSeq Adapter, Index 5 (100% over 35bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC279520.6597536356433129Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA269230.6354660536786245TruSeq Adapter, Index 8 (100% over 40bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC242500.5723749879919268No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG238850.5637598593066875Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC229500.5416909680171018No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG212970.5026750564644975No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA204130.48180992288161656Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC201430.47543708796376827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT190510.4496625111849154Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC169880.40096933179409705No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC168830.3984910071038227Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG162060.3825117136246254No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC157800.3724567963097981Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC139520.3293103436067366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC133130.31422796763449573No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT123150.29067208153074553Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG121940.28781610736385793TruSeq Adapter, Index 8 (100% over 38bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG119910.2830246796293276No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC116230.27433874166722333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA106430.25120771122466296No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG105820.24976792259507502Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG105370.24870578344210031TruSeq Adapter, Index 8 (100% over 38bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC101070.23855645375811976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC100560.23735269605141507No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT99800.23555886103750223TruSeq Adapter, Index 8 (100% over 37bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC99540.23494518063800576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC95410.22519710352292677Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC93430.22052369124983803TruSeq Adapter, Index 5 (100% over 35bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG91830.21674719648370572No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC91150.2151421862080995No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC90470.21353717593249327Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA89330.210846423411624No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC85840.2026089442029979TruSeq Adapter, Index 5 (100% over 35bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA83390.19682618659235784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC78000.18410411984894964No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG75270.1776604756542364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA73170.17270382627368774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT71520.16880931604611385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG70950.16746393978567922Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA70690.1668502593861827TruSeq Adapter, Index 8 (100% over 40bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT70650.1667558470170294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA70500.1664018006327045Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC69390.1637818573887002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC68620.16196441928249902Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA66550.15707857917881538Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC64970.15334929059725974Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC64930.15325487822810643Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT64500.15223994525970838Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG61610.1454186515883819No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC61100.14421489388167721Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC60980.14393165677421732Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA59580.14062722385385154Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA56280.13283820339870367No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC53910.1272442705263702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC53590.12648897157314373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC53020.1251435953127091Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC52460.1238218221445628Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC52110.12299571391447137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA51570.12172114693090172No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT51550.12167394074632505No Hit
TCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAG49990.11799185834934607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA49970.1179446521647694No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC49540.11692971919637135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC48940.11551353365907174No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT48690.11492345635186357TruSeq Adapter, Index 8 (100% over 36bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC48350.11412095121406045No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG47480.11206748218497602Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA47230.11147740487776785No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC44950.10609589983602932Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC44860.10588347200543437No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC44460.1049393483139013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAG44370.10472692048330637No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC43890.10359397205346667No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC43860.10352316277660169Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC43740.10323992566914177Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA43200.1019653586855721No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC43120.10177653394726549No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC43070.10165851848582387No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA42410.10010071439479429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)