FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500153
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2957678.45011632348643Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1045432.9868122907770034No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC839252.397752326826856No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC753912.1539344137242002Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC743592.1244499883290815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC487211.3919677225538427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA410711.1734058482586331No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA365861.045268592544383TruSeq Adapter, Index 7 (97% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG329340.9409302964756111No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC286680.8190499101039297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA264200.7548241462587493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA216280.6179158453930442TruSeq Adapter, Index 9 (100% over 40bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT193360.5524329936434208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG188910.5397192637007583TruSeq Adapter, Index 9 (100% over 49bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC187240.534948043699804No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC186930.5340623681307646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC179240.5120918999826579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG173410.4954354852487877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC164120.4688937883572518No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC154270.44075216140551565Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA122790.3508132358785459TruSeq Adapter, Index 9 (100% over 38bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA121600.34741338450062037Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT118540.338670909528812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC114520.3271856973109461No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC111430.31835751180019844TruSeq Adapter, Index 9 (100% over 37bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC111340.31810038018338055Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC99890.28538752448821525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC98280.2807877255651396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA87850.2509890281939104TruSeq Adapter, Index 7 (97% over 35bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC86400.24684635214517764Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA85080.24307508843184855TruSeq Adapter, Index 9 (100% over 38bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC83390.23824672807160144Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC80480.22993280579448958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG78240.22353308555368864Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA76600.2188475760916737No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG76560.21873329537308797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA76200.2177047689058164No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA74670.2133335314199122TruSeq Adapter, Index 7 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC74540.21296211908450857No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC73900.21113362758713694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC69660.19901987141704947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC68880.19679139740462775No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG67540.19296299333200578No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC67040.19153448434968415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG64690.1848204921327725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG64380.18393481656373306Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT61780.1765065698556606Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC61710.17630657859813556Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC61240.17496378015475325No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG60730.17350670099278517No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA60570.17304957811844227No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT58980.16850691955465946No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC56430.16122152374481916No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA54970.15705027751644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC54210.15487894386331114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA54090.1545361017075539Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT52530.15007915368271044TruSeq Adapter, Index 9 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC52380.14965060098801394No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA52240.1492506184729639No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC51780.147936390209228No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT50600.14456510901094896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT49600.1417080910463057Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG49000.13999388026751972No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC48050.13727971320110863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA47120.1346226864939904No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG46880.133937002182476No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG44240.12639447475581783No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA43690.12482311487526403No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC43530.1243659920009211No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA43520.12433742182127466No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC43300.12370887786905314Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA43230.12350888661152813TruSeq Adapter, Index 9 (100% over 40bp)
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT43080.12308033391683164Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG42570.12162325475486357No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT42510.12145183367698498No Hit
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA41750.11928050002385611No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC41420.11833768409552382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG41390.11825197355658454Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA40070.11448070984325542Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC39970.11419500804679111Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA39660.11330933247775168Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA39530.11293792014234806Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC39530.11293792014234806Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC39420.11262364816623731Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT39330.11236651654941941Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG38090.10882381427326177No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC37420.10690961223695078Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG36480.10422401535018612Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA35980.1027955063678645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)