FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4397270
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2382035.417065588421907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1793774.079281008443876No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1136262.5840123531190944No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC765111.7399659334086832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC675251.5356118682728146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC661811.5050474498950484Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG647981.4735961175911418No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA516931.1755702970251996No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC433960.9868850445844808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC392390.8923491166109881No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA366730.833994728547485TruSeq Adapter, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG299420.680922481448717Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC265940.6047843320969601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA263730.5997584865154971Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA252420.5740379826574216TruSeq Adapter, Index 10 (100% over 40bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG250120.5688074646314646No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT207930.4728615709292356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA204880.4659254491991622Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG190530.4332915649937348TruSeq Adapter, Index 10 (100% over 49bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG185650.42219377022561727No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC180250.40991342355597904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC180200.4097997166423713No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC178970.40700252656762037Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC172640.3926072313048778No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC160110.36411227875477287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC158150.3596549677413486No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT152680.34721543139265953Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC137930.31367189187836997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG129390.29425075103416437No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT128950.2932501301944161TruSeq Adapter, Index 10 (100% over 38bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC126010.2865641636742797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT125690.28583643942719006TruSeq Adapter, Index 10 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC118030.268416540262481No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC117650.26755236771906205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG109570.24917733048004784Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC99800.2269589995610913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC97690.2221605678068438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA95800.2178624464724704No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA95160.21640699797829108TruSeq Adapter, Index 7 (97% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG91220.2074468931859995No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG90310.20537742735833825No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT89620.20380827195055112Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC87880.19985127135700104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT87590.19919177125807602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC87100.19807744350471998TruSeq Adapter, Index 10 (100% over 37bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA84320.19175533910812845TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA80900.18397778621735758No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT79630.18108963061172045No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT79600.1810214064635558Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC78230.17790583703070315No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC78190.17781487149981695Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA77840.1770189231045626No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA77650.17658683683285312Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG74080.16846816320125896Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC73310.16671707673169944No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC72720.16537533515112787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA72450.16476131781764594Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC71250.1620323518910597Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT70590.16053142063143724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT68740.15632426482795006Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA68290.1553009026054802No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC66490.15120745371560082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT64660.14704578067755675No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA64650.1470230392948352Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC63970.14547662526976965Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC61540.13995046926843246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC61040.13881340013235485Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC60880.13844953800881No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA60310.13715327919368153TruSeq Adapter, Index 10 (100% over 40bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA60130.1367439343046936No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA59240.13471995124247543No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT59230.1346972098597539Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC58280.13253677850120643No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC58140.1322183991431047No Hit
GAGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGG56950.12951217459923997No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG55860.1270333638825908No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG55510.12623741548733647No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC55510.12623741548733647Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG55230.125600656771133No Hit
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC53860.12248508733828034Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC53310.12123431128859498Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA52590.11959693173264321No Hit
TCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAG52360.1190738799300475No Hit
ACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTAGA51870.11795955217669145No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG51550.11723182792960178No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC49770.11318386180516549Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA49350.11222872373086029No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT48760.1108869821502887Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG47800.10870380940901968TruSeq Adapter, Index 7 (97% over 36bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC47540.10811253345825933Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC45660.10383715350660752No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG45550.10358699829667044No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAT45020.10238170501242817No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC44930.1021770325679342Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT44150.10040320471565313No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC44110.10031223918476691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)