FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10420784
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTATGCCG7425577.1257306551983035TruSeq Adapter, Index 4 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTA6285366.031561540859114TruSeq Adapter, Index 4 (97% over 38bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2583642.479314416266569No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2372712.2769016227569825Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2195032.1063962174055235No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1674531.606913644885068No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1091061.0470037571069508Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC807420.7748169427559386No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG728740.6993139863564968No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC712350.6835858031411073Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG565700.5428574280015784No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC534150.5125813950274759No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG492160.4722869219820697No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT447140.42908479822631385TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC430640.413251056734311No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGA428490.41118787223686815TruSeq Adapter, Index 4 (97% over 38bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC418890.4019755135506119No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA399350.3832245251412945Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC382320.3668821846801546No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG355850.341481024844196No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC346520.3325277637459907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA329610.31630057776842896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG317960.30512099665437836No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG279850.2685498519113341No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC275280.26416438532839753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT254070.2438108303559502No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC247200.23721823617109805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA233240.22382193124816713No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA230680.2213653022651655No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGT230470.2211637819189036TruSeq Adapter, Index 4 (97% over 38bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC220690.21177869150728004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA215410.20671189422983913No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG214690.20602096732836991Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA213550.204926999734377No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC210640.20213450350760556No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG210530.20202894523099224No Hit
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA209340.20088699660217504No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC208040.1996394896967445No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA207480.1991021021067129No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA201190.1930660879258221No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT193290.1854850844235904No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACC192080.1843239433808435TruSeq Adapter, Index 4 (97% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC179100.1718680667404679No Hit
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA168680.16186881908309395No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC164580.15793437422750534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACCTATGATGATGACTGGTGGCGTATGAGTCATTGACGGTGAATACAGA163530.15692677249619605No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC161910.15537218696789032Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG159960.15350092660974451No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG158240.15185037901179027No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCG153570.14736895035920522No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGA145030.13917378961122312No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC140370.1347019571656029No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT134490.12905938747027096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG133760.12835886436183688No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA130480.125211308477366Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC130450.12518251985647147No Hit
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA129920.1246739208873344No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA122730.11777424807960706No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT119550.11472265426478469No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA119080.11427163253743672No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATC115460.11079780561616094No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG115310.11065386251168818No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG111790.10727599766006089No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC111030.10654668593073227Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGT110450.10599010592677097TruSeq Adapter, Index 4 (97% over 38bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG109300.10488654212581318No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC109270.10485775350491863No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG107750.10339913004626139No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC105820.10154706210204531No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT104710.10048188312894692TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)