FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11620881
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTA120983910.410906023390137TruSeq Adapter, Index 13 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTATGCCG4951324.260709665644111TruSeq Adapter, Index 13 (97% over 34bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3039152.6152492224987074Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG3005652.5864218039923137No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2988612.571758543952046No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2184601.8798918945990413No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1179581.0150521290081191Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC928930.7993628021834145No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG719510.6191527131204596TruSeq Adapter, Index 2 (97% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG718560.6183352191628156No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC645920.555827049601489Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC631150.5431171698600132No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC576370.4959778867023938No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG575830.49551320592646975No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC477110.41056267592792667No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACC474170.4080327472590073TruSeq Adapter, Index 13 (97% over 38bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC451230.38829241948179316Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGT429930.3699633444314592TruSeq Adapter, Index 13 (97% over 38bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA410030.3528389973187058Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG345330.2971633562033722No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC332800.2863810411620255Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG322260.2773111608319541No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA309100.2659867182186961No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT300650.2587153245954416No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC299990.25814738142486787No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA291830.2511255385886836No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG285400.2455923952753668Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC276040.23753792849268485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA271820.23390653428083463No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT267940.23056771685382543TruSeq Adapter, Index 13 (97% over 38bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA266500.22932856811802824No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC263870.2270654006352875No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT263410.2266695614557967Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG249610.21479438607107326No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGA240060.20657642049686248TruSeq Adapter, Index 13 (97% over 38bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG231980.19962341925711138No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA230890.19868545250570935No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC226740.19511429469073815No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTA226300.19473566591035568TruSeq Adapter, Index 13 (97% over 38bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA221330.19045888173194442No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC218800.18828176624474513No Hit
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGT205290.17665614164709198TruSeq Adapter, Index 13 (97% over 38bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC197740.1701592159837107Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA186900.1608311796670149No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC184900.15911013975618546Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG182640.15716536465694814No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA177100.15239808410395045No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA166030.1428721281975093Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC165280.14222673823094822No Hit
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA163610.14078966990540562No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC162500.13983449275489526No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC159750.13746806287750474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC159640.1373734056824091No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC157860.13584168016177087Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC155340.13367316987412572No Hit
GATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAG154680.133105226703552No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGA154550.13299335910934806No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT150600.12959430528545984No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG149600.12873378533004512No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG148930.12815723695991724No Hit
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA148720.12797652776928015No Hit
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGT145920.12556707189411886TruSeq Adapter, Index 13 (97% over 38bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGA142540.12265851444481705No Hit
TCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGA141620.1218668360858355No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG141590.12184102048717306No Hit
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA140160.12061047695092998No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG137180.11804612748379405No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC136960.1178568130936028No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT136920.11782239229538621No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA135580.11666929555513045No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC133330.11473312565544729Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG133050.11449218006793116TruSeq Adapter, Index 2 (97% over 35bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCG126940.1092344031403471No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT124950.10752196842907177Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA123330.1061279261012999No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA118870.10229000710015015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)