FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340191
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63406810.00077127013997Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1955253.0838976302133485Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1134511.78939404191451No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT950811.499655136572384TruSeq Adapter, Index 1 (100% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC883701.3938065903692807No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG719041.1340983260598931No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC570710.9001463836026392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA543140.8566618892080695TruSeq Adapter, Index 27 (97% over 39bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC467130.7367759110096209TruSeq Adapter, Index 27 (97% over 39bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA440640.6949948353290933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC440080.6941115811810717Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC329920.5203628723487983TruSeq Adapter, Index 1 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCG319460.5038649466553926TruSeq Adapter, Index 27 (97% over 35bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG297190.46873982187602864Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC268050.4227790613878983No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT263500.41560262143522175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC239520.3777804170252915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC236850.37356918742668793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC225830.3561880075852604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT216620.3416616313294032TruSeq Adapter, Index 1 (100% over 35bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC212100.3345325085632278No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC195870.3089339106660982Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC191470.30199405664592754Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG183510.28943922982761877No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC180190.28420279452149Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA178280.28119026698091587No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA175320.27652163791280104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCGGGGC174400.27507057752676534No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC174330.2749601707582627No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA170540.2689824328636156No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT167480.26415607984049694TruSeq Adapter, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC164230.2590300513028708Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT149730.23616007782730838Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA149480.23576576793979867Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA149070.23511909972428274TruSeq Adapter, Index 1 (97% over 37bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC147090.23199616541520596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC146470.23101827689418192No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC146210.23060819461117182Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG141660.2234317546584953No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA133710.2108927002356869Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG131390.20723350447959693No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC129870.20483610036353797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG128420.2025491030159817No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC123650.19502567036229665TruSeq Adapter, Index 1 (97% over 38bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA123280.1944420917287823Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC119770.18890598090814614No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT114820.18109864513545412Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC103260.1628657559370057No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC102900.16229794969899172No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA101640.16031062786594283Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC101290.15975859402342926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG99800.15740850709387147No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG97800.15425402799379387Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC97630.15398589727028728No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT97210.153323456659271No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC92050.14518490058107084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC89950.14187269752598936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT87260.137629923136385Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG86600.13658894503335942No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC85890.13546910495283185TruSeq Adapter, Index 27 (97% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA85750.13524829141582645No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC83600.13185722638324304Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG83560.13179413680124147No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA81450.12846616135065964Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA79190.12490159996757196No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA78560.12390793905104752No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC76750.12105313546547729Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT70290.1108641679722267No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA70030.11045408568921662No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC69500.10961814872769607TruSeq Adapter, Index 27 (97% over 39bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC68670.10830903990116388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA68250.10764659929014757Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA67090.10581700141210257No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT65340.10305683219953468TruSeq Adapter, Index 8 (97% over 36bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG64640.10195276451450752No Hit
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT63510.10017048382296369TruSeq Adapter, Index 1 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)