FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002433073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615126
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3821108.279513928763809Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTA1200102.600362373638336TruSeq Adapter, Index 22 (97% over 37bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1034632.2418239502020096Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG954212.06757085288679No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA827381.7927571208240036Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC742711.6092951741729262Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC707061.532049179155672Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC585201.2680043838456416Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC434410.9412744094094072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATAC396900.859998188565166TruSeq Adapter, Index 22 (97% over 37bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC358650.7771185445424459Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG347800.7536088938850206TruSeq Adapter, Index 2 (100% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC333830.7233388644210365No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG318060.6891686164148064No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG275440.5968201084867455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT274630.595065010142735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC253860.5500608217413782Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC247690.5366917392937918Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC219520.47565331910764735Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA217160.47053969924114747No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC189860.4113863846837551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA164830.35715167906575035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAT164800.3570866754233795TruSeq Adapter, Index 22 (97% over 37bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC164630.35671832144994525No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT162690.352514752576636Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC144900.3139675926507749Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAT139310.3018552472890231TruSeq Adapter, Index 22 (97% over 37bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC136640.2960699231180254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT131990.28599435855055744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG126130.27329698040746886No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG115920.2511740741206199Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATAC113970.24694883736652043TruSeq Adapter, Index 22 (97% over 37bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG113370.24564876451910522TruSeq Adapter, Index 2 (100% over 35bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT109320.23687327279905251Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA107470.2328647148528556Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG103070.223330847305144Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA102640.22239912843116308Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT102440.22196577081535804Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC101370.2196473075708009Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG99910.21648379697542386TruSeq Adapter, Index 2 (100% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTATGCCG99750.2161371108827798TruSeq Adapter, Index 22 (96% over 33bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC97930.21219355657895364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC96780.2097017502880745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG95390.20668991485822924Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC93940.20354807214364246Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC93920.20350473638206193Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA87240.18903059201417252TruSeq Adapter, Index 22 (97% over 37bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC87000.18851056287520646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC86770.1880122016170306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC83690.1813384943336325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC82650.1790850347314461Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC81650.17691824665242076Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC79060.17130626552774508Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT73930.16019064268234495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA72720.1575688291067243Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC71680.15531536950453792Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG71440.15479534036557183No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA71060.1539719608955422No Hit
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGA68550.1485333228171885TruSeq Adapter, Index 22 (97% over 37bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC67650.1465832135460657Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC67530.14632319897658266Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC66540.14417807877834754Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC64380.13949781652765278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC63070.13665932414412954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC62300.13499089732328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT61150.13249909103240085Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC59990.12998561686073143No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC59730.12942225196018484No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC56610.1226618731536257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA55410.12006172745879529Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCT54890.11893499765770207TruSeq Adapter, Index 22 (97% over 37bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG53500.11592316222785683Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC53080.11501311123466619Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC52480.11371303838725096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATA51130.11078787448056673TruSeq Adapter, Index 22 (97% over 37bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC50330.10905444401734644Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC49890.1081010572625753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC49680.10764603176597995Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATAC49350.10693099169990158TruSeq Adapter, Index 22 (97% over 37bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA49230.10667097713041854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA48450.10498088242877875TruSeq Adapter, Index 2 (100% over 36bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA47770.10350746653504152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG46750.10129734269443565Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATAC46530.10082064931705008TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)