FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238192
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2422723.8836893766655467Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2246663.6014601666636747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTA2233803.5808452192558358TruSeq Adapter, Index 22 (97% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1762212.824872976016128No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1648312.642288021914042Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC1298882.082141748763103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC970861.5563163172919332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC938671.504714827629544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC864681.38610674374883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC695741.1152910971640502No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACT578180.9268390584964361TruSeq Adapter, Index 22 (97% over 37bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG563610.9034829322342114No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT443620.7111355341419436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC440840.7066791147178542Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG419960.6732078781800881No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC361850.5800558879880581Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC335020.5370466314598845No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC292240.4684690692431397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC287220.4604218658226614Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA284310.4557570526844958Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA278060.4457381241231434Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG261850.4197530310064198Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC257950.41350121958413594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC252230.40433189616478615Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG248790.3988174778846178No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG247220.39630072303000613TruSeq Adapter, Index 2 (100% over 35bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC243260.38995272989353325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT241810.3876283384672995Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC239360.3837009184712494No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT236470.37906816590448Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCTCGTATGCCG231940.3718064464832118TruSeq Adapter, Index 22 (96% over 33bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC231840.37164614362623016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG187440.3004716751263828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA184500.2957587711311226Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC165050.26457986544819395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG163180.2615822020226373Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT158100.2534388168879701Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC156080.25020069917694104No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC154970.2484213374644448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC154530.2477160048937256Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC154430.24755570203674396Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG152270.24409316032594058No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC151980.2436282820406938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG147950.23716807690433384Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC147410.23630244147663299Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC144560.23173381005265628Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT142180.22791860205649328Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAA139780.224071333488934TruSeq Adapter, Index 22 (97% over 37bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC137340.220159943778582Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC136750.21921415692239032Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC133020.21323486035697525Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC125220.20073123751240743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC122470.19632290894541238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT122240.19595421237435462Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC121310.19446339580442537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAA118530.19000697638033584TruSeq Adapter, Index 22 (97% over 37bp)
CGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACTAATCT117250.18795509981097086TruSeq Adapter, Index 22 (97% over 37bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA117190.18785891809678187Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC116570.18686504038349575No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC113580.18207198495974475Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC112010.17955523010513302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA104960.16825387868792752No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACT100090.16044712955292176TruSeq Adapter, Index 22 (97% over 37bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC99730.15987003926778784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC98840.15844334384065126No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC96910.15534949870090564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG94070.15079689756262712Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC91790.14714199242344578Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC91670.1469496289950678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC87690.1405695752871986Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG86160.13811694157537954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG84380.13526355072110638TruSeq Adapter, Index 2 (100% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG81950.13136819129645255No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG79790.12790564958564918TruSeq Adapter, Index 2 (100% over 35bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACT78900.1264789541585126TruSeq Adapter, Index 22 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG78810.12633468158722913No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG78100.1251965313026595No Hit
GTAGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAACT77780.12468356216031826TruSeq Adapter, Index 22 (97% over 37bp)
AAAGTGCTGCGACATTTGAGCGTAGATCGGAAGAGCACACGTCTGAACTC77750.12463547130322375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACAGTAGTCTGCACATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCA77360.12401029016099537Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT74290.11908899245165908Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGACTCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC72850.11678063131112348TruSeq Adapter, Index 11 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG72370.11601117759761162No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG71140.11403945245673747No Hit
TGAGATGAAGCACTGTAGCTCTTAGATCGGAAGAGCACACGTCTGAACTC71030.11386311931405767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC70640.11323793817182928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA68860.1103845473175561Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC67350.10796397417713337Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT66280.10624873360742984Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC65510.10501440160867123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA64990.1041808267523667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC64010.10260985875394665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC63860.10236940446847419Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC63340.10153582961216968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTGCTGTCATAGCTGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC62660.10044577018469454Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)