FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4311700
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1884274.370132430363894Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1654963.8383004383421855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1633523.7885752719345036No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTA1202702.7893870167219426TruSeq Adapter, Index 7 (97% over 36bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC1106502.5662731637173275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC932252.162140223113853No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC694341.6103625020293622Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA652221.512674815038152Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA590981.3706426699445695Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG580841.3471252638170559No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC475451.1026973119651182Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC471781.0941855880511169Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC414620.9616160679082497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC363720.8435651831064314Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATT360500.8360971310619941TruSeq Adapter, Index 7 (97% over 36bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC341160.7912424333789457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT329850.7650114803905652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG275390.6387039914650834Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG230880.5354732472110768No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC230490.5345687315907879Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC224290.5201892524990143Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA210820.4889486745367257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG198240.45977224760535285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC184420.42771992485562543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC177260.4111139457754482Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG168990.39193357608367935TruSeq Adapter, Index 2 (100% over 35bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG168790.3914697219194285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTATGCCG148920.34538581070111557TruSeq Adapter, Index 7 (96% over 32bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT137370.3185982327156342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC136590.31678920147505624Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC133510.3096458473455945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC131510.30500730570308693No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC127370.2954055245030962Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC122690.2845513370596285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA119670.27754713917944196No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA113890.264141753832595Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC112930.2619152538441914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT109720.2544703945079667Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC107320.24890414453695758Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC105610.24493819143261356Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC98120.22756685298142265Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA95820.2222325300925389No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG94760.2197741030220099TruSeq Adapter, Index 2 (100% over 35bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA89390.20731961871187699Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCA87870.2037943270635712TruSeq Adapter, Index 7 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC87610.2031913166500452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC86750.20119674374376698Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA85380.19801934271864927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT85350.19794976459401162Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG81970.19011062921817382No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC78240.18145974905489715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC77850.18055523343460816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC76920.17839831157084213Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC75580.17529048867036204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC74830.1735510355544217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC72790.16881972307906395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC71700.1662917178838973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC69830.1619546814481527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC69480.16114293666071386No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC64840.15038152005009625Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATT60620.14059419718440522TruSeq Adapter, Index 7 (97% over 36bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG60430.140153535728367Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATACAAGGGCAAGCTCTCTGTAGATCGGAAGAGCACACGTCTGAACTCC60220.13966648885590371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC60210.13964329614769116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC60050.13927221281629054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA59550.13811257740566366Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG59320.13757914511677527TruSeq Adapter, Index 2 (100% over 35bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT59000.13683697845397405Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG58990.13681378574576153Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGCAGATCGGAAGAGCACACGTCTGAACTC58490.13565415033513462Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT57880.13423939513416983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCA57440.13321891597281815TruSeq Adapter, Index 7 (97% over 36bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG57270.132824639933205Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC56270.1305053691119512Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT53920.12505508268200477Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC51020.11832919730036875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC51010.11830600459215622Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA50980.1182364264675186No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT49640.11512860356703854Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA49400.11457197856993762Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA49320.11438643690423732Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGATAGATCGGAAGAGCACACGTCTGAACTCC48650.11283252545399726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC47840.11095391608878169Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATT47560.11030452025883061TruSeq Adapter, Index 7 (97% over 36bp)
TGGCTCAGTTCAGCAGGAACAAGATCGGAAGAGCACACGTCTGAACTCCA47300.10970150984530463Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC47220.10951596817960432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG46530.10791567131293921No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT45770.10615302548878632Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTAGAGGGAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC45640.10585152028202334Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA45200.10483104112067167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTCTGAACTCCAG45050.1044831504974836Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)