FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9235129
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8540119.247418200655346Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2631572.849521647180023Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2401812.600732485707563No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1464421.5857060578146769No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1431921.5505143458201829No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1189831.2883739902279654TruSeq Adapter, Index 1 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA1162731.259029516534095TruSeq Adapter, Index 13 (97% over 38bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC1158261.2541893026074675No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCCG562470.6090548383244023TruSeq Adapter, Index 13 (97% over 34bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC549390.594891527774003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC502330.5439339288059756No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG496220.5373178869510107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA485190.5253743613110331TruSeq Adapter, Index 13 (97% over 38bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC483890.5239666928312533No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA426780.46212673369262086TruSeq Adapter, Index 13 (97% over 38bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC343710.372176717834694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT336690.3645753080438833Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC335620.3634166886028338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA333140.36073129027217704No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG326910.3539853097883094No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC323440.350227917769205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA312620.33851178472980725No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG304440.32965430152626995No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT302930.32801924044591035TruSeq Adapter, Index 1 (100% over 35bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC300950.32587525306901505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC299650.3244675845892353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT294980.3194108062811034TruSeq Adapter, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC287460.31126798553653123No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG267050.289167590403989No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG259470.2809598003449654No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC259400.28088400281143877No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA249930.270629679347197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC237570.257246000570214No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA235130.25460391511585817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC228660.24759805737418505No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT223090.2415667393492825Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA213090.23073852027405356No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT205880.2229313743208135Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC192410.20834576322648013No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC189380.20506481284668573Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA184050.1992933720795887Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA182690.1978207342853576No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC179480.1943448759622091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG177360.19204929351826053No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC171050.18521668728179108No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA168130.18205484731182422No Hit
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC166530.1803223322597876Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA165540.17925033857133993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA160460.17374960328112365Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC158930.17209288576261358Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG158800.17195211891463563No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC154760.16757751840824314Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT153160.1658450033562065No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA146620.15876334808100678No Hit
CAACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTA142200.15397727524975557No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC142110.15387982127807853TruSeq Adapter, Index 13 (97% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC137060.1484115706450879No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC134390.14552043615200178No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA127950.13854706306755435Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGG127780.13836298334327543No Hit
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG123110.13330620503514354No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC122980.13316543818716553No Hit
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA119310.12919148178655654No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT117990.1277621568686263No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC117810.1275672489252722No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAGATCGGAAGAGCACACG117100.12679844537093093No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCA116940.12662519386572726TruSeq Adapter, Index 13 (97% over 38bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA114640.1241347034784246No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA114530.1240155930685971No Hit
GATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAG112720.12205568541598065No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG112280.12157924377667058No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCG110040.1191537227038193No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA109310.11836326271132758No Hit
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA105700.11445427562516994Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC104450.11310074824076631Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC104130.11275424523035898No Hit
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC103600.11218034961937184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA102610.11110835593092419No Hit
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCAC98660.10683120939620876No Hit
AGTGTGAGTCTGAAACCAATTTTTTGTAGGCCTTGCGTTTCTTAGCAGGG95380.10327955353953365No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC95190.10307381737710432Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)