FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002433114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6074338
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA61297310.09119018401676Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1534462.526135358289249Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTA1488862.45106544943663TruSeq Adapter, Index 8 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1442112.3741023301633857No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1197961.9721655265149882No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1159381.908652432577838No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC746981.22973071304231TruSeq Adapter, Index 5 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCCG699111.15092377144637TruSeq Adapter, Index 8 (96% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC660271.0869826473271655Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCG481170.7921357026889185TruSeq Adapter, Index 8 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC462340.7611364398885936Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG303820.5001697304298839No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA282840.46563098727795527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT238450.3925530650418202Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC225690.3715466607225347Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG221030.36387504284417493No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC211770.3486305832833142No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG210830.3470830895481944Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA208320.3429509520214384No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA206000.3391316057815683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG199870.3290399711046702TruSeq Adapter, Index 8 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC191800.3157545727616738Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC191770.3157051846637444No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC191050.3145198703134399Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC189930.31267604799074405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC186930.30773723819780857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA180220.29669076696094293No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC172640.284212040884126Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCG164710.27115712033146655TruSeq Adapter, Index 8 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC164140.27021874647080885No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC153740.2530975391886326TruSeq Adapter, Index 5 (97% over 35bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTC142080.23390203179342342TruSeq Adapter, Index 8 (97% over 36bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA138800.228502266419814No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC123780.2037752920565171Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA118830.19562625589817356No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT118720.19544516620576594No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC118460.19501713602371157No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC116650.1920373874486405No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC112970.18597911410263968TruSeq Adapter, Index 5 (97% over 35bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA112080.1845139338640688Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC109420.18013485584766603No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC107440.1768752413843286Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG106580.17545944924368714No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA106340.1750643444602523No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT104960.17279249195550198Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCG96900.1595235563118154TruSeq Adapter, Index 8 (97% over 36bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC96840.15942478011595668No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA96480.15883212294080443No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT94780.15603346405814097Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC90200.14849354777425952No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT89930.14804905489289533Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC81190.13366065569614335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG77560.12768469584669145No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG76490.12592318702054445Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG76460.1258737989226151No Hit
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC75630.12450739487990295Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA75580.12442508138335404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCAC73180.12047403354900567No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC72440.11925579380008158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT68750.11318105775477097Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC68380.11257193788030892Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC67930.1118311164113686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC67770.11156771322241206Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC65110.10718863520600928Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAG64550.10626672404466134No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGA63590.10468630491092198No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGG62730.10327051277028047No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG62330.10261200479788908Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT62150.10231567621031296Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA61520.10127852615379651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)