FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002433124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593576
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1424825.493650465611958No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1200154.627394763060732Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA1137994.387725672970447TruSeq Adapter, Index 27 (97% over 39bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC749852.891181904829471Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC669612.581802114146645Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC585782.258580431034217No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC473361.8251248469294903Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG417851.6110960311168827No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA357351.377827370395161Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC295081.1377341554672005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC263391.0155476454131285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC260561.0046360700438315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC249030.9601800756947165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA247980.9561316113350833Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT229400.8844930705712885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC226580.8736200520054164Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC224630.8661014753375263TruSeq Adapter, Index 27 (97% over 39bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG182640.7042014577556239No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC172850.6664543472024725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC136490.5262618099488892Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC130680.5038603071589187Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC123700.4769476583682144Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG122940.4740173413079084Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT121390.4680410367770214Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG110920.4276720635909647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT96470.37195748264172707TruSeq Adapter, Index 1 (100% over 35bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC93850.3618556001443567Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA92880.3581155902121241No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT83530.32206497900967623Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA80710.31119196044380426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC80010.3084929842040488Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC75480.2910267522524885No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG74990.28913746888465963Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC68660.2647310123165853Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC65500.2525470624342606Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT61640.23766413631218058TruSeq Adapter, Index 1 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC59900.23095525251621696Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC57380.22123893805309736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC55610.21441438384685857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA55160.21267932769273No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC54570.21040447629065043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA53740.20720426160636898Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA52620.20288589962276024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG52560.20265455880220978No Hit
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC52240.20142074109260727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT52070.20076527543438097Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC51870.19999413936587937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC51600.1989531056734023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC51110.19706382230557348TruSeq Adapter, Index 1 (97% over 38bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA50470.19459618688636848No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC49940.19255267630483935Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA48740.18792585989382998Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC46750.18025305601223948Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC44860.17296582016489975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC44600.17196334327584772Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC42790.1649845618559086No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC42540.16402064177028164Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC40770.15719608756404285Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC40100.15461278173456264Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC38180.14720987547694767Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC38120.14697853465639718Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG38050.14670863703242165No Hit
AAAGTGCTGCGACATTTGAGCGTAGATCGGAAGAGCACACGTCTGAACTC37950.14632306899817088Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT37680.14528203530569378Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC37080.14296862710018907Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT35050.13514159600489825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA34800.1341776759192713Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC34290.13221127894459234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC32990.1271988944993322TruSeq Adapter, Index 27 (97% over 39bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC32900.1268518832685065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGCAGATCGGAAGAGCACACGTCTGAACTC32680.12600363359315478Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG32160.12399867981505072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA32130.12388300940477548Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTAGAGGGAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC31870.12288053251572346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG31720.1223021804643473Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT31260.12052856750679371TruSeq Adapter, Index 1 (100% over 35bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT30870.11902485217321566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATACAAGGGCAAGCTCTCTGTAGATCGGAAGAGCACACGTCTGAACTCC30690.11833082971156428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCG30680.11829227290813919TruSeq Adapter, Index 27 (97% over 35bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC30030.11578608068550911TruSeq Adapter, Index 1 (97% over 38bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGT29820.11497638781358248TruSeq Adapter, Index 27 (97% over 39bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT29290.11293287723205335Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG28770.11092792345394929Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTCTGAACTCCAG27850.10738069753884211Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC27750.10699512950459135Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA27600.10641677745321518Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA26990.10406481244428542Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG26980.10402625564086034Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC26190.10098026817027918TruSeq Adapter, Index 27 (97% over 39bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA26130.10074892734972872Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTACAAAGGAAAGCGCTTTCTGTAGATCGGAAGAGCACACGTCTGAACTC26110.10067181374287856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)