FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7296813
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA79609110.910119253432972Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2328333.190886212926109Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1325761.8169028040049815TruSeq Adapter, Index 2 (97% over 35bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1276811.7498187222284578Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA986231.3515900708980757No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC758281.0391934122472373Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG755161.0349175729184783No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC738661.0123049610836952No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG590680.8095040944587727TruSeq Adapter, Index 4 (100% over 49bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA498050.682558262079623TruSeq Adapter, Index 4 (100% over 40bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG488910.6700322455844764Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC457480.6269586461925227Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC440340.6034689391108146TruSeq Adapter, Index 4 (100% over 38bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA401540.5502950397659909Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC353250.4841154624628588TruSeq Adapter, Index 4 (100% over 50bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG303020.4152771901924854TruSeq Adapter, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT298170.4086304527743825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC291420.3993798388419711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA281650.38599043171313285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC270670.3709427663830771Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG263360.3609246941096065No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT225290.3087512315308067Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC216300.29643078423415814Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC211600.2899896160145532No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC210030.2878379917369405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195000.267239958047438Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC173890.23830951951214868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC170700.23393774789075725Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG163280.2237689248717214Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG162380.2225355096807332TruSeq Adapter, Index 2 (97% over 35bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT161320.22108282067801382Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC161120.22080872841334975Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC157240.2154913384788674TruSeq Adapter, Index 4 (100% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG154640.2119281390382349No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC152780.20937908097685937No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC152030.20835123498436922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC151180.20718634285954704No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT150900.2068026136890174Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC147380.20197858983093028No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA145160.19893616569315944No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC140280.19224831443535692TruSeq Adapter, Index 4 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA137770.18880845651382322No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA137270.18812322585216312Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC133090.1823946975206847Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC132190.18116128232969653No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC131490.1802019594033724Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA130430.178749270400653Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC127160.17426786187339596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA126170.17291110516330896No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT120710.1654283863379807Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA112010.15350537282509502Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT104300.1429391160222963Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA102690.1407326732917508No Hit
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC99590.1364842431894582Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG97960.13425039123244628No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC97680.1338666620619166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG97140.13312661294732372Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC96390.13209876695483355Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC92130.12626060171748954Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC90150.12354708829731556No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC87690.12017575344194789No Hit
AACCCGTAGATCCGAACTTGTGCAGATCGGAAGAGCACACGTCTGAACTC87300.119641273525853Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT87230.1195453412332206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC84320.11555729878235882No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG84200.11539284342356039No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA83030.11378940367527578TruSeq Adapter, Index 4 (100% over 40bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC79130.10844460451432701Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA77530.1062518663970147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA76120.10431951593113321Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC76000.1041550605723348Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT75680.10371651294887234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG75530.10351094375037431Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC74830.10255162082405016Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC74310.10183898093592367Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG73960.10135931947276161TruSeq Adapter, Index 2 (97% over 35bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA73210.10033147348027145TruSeq Adapter, Index 2 (97% over 36bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG73080.10015331350823983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)