FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11052418
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5490124.967347416646747Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2789762.5241173469914004No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2081071.8829092421224027Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1649761.4926688440484246No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC1384791.2529294494652665Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT1347821.2194797554707033TruSeq Adapter, Index 1 (97% over 35bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1335761.208568116044833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1328671.2021532301800384No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC1113891.0078247131080276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1032660.9343294833763979No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1011830.9154829287129749Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG1009080.912994785394472TruSeq Adapter, Index 20 (97% over 49bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG804040.7274788195669039Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA713660.6457048584300739Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT702450.6355622814844679Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC622250.562998974523041Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC594520.5379094420786474Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCA591160.5348693833331313TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCCGTC587210.5312955047483727TruSeq Adapter, Index 20 (98% over 50bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC501570.45381019791325306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC472910.42787922063751116Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC453520.4103355482935951Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA450400.40751263660133014Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG428260.3874808209389113No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT425830.38528220702474336Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC420340.38031496818162325No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC363200.32861587392007796TruSeq Adapter, Index 20 (100% over 38bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC354670.32089810573577654Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT342640.3100136096915625Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC341090.3086112016393155TruSeq Adapter, Index 20 (100% over 38bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT302690.273867673119131TruSeq Adapter, Index 1 (97% over 35bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG299260.27076427981641665Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCA296570.26833042326122664TruSeq Adapter, Index 20 (97% over 39bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG286710.2594092984901584TruSeq Adapter, Index 20 (100% over 37bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC286180.2589297654142288No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA267310.2418565783523569No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG263950.23881651960684078Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC256480.23205781757439864No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA244560.2212728472629247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC242010.21896565982213123No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC236240.21374508275021809Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC220450.19945861620506933No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC219260.19838192873269903Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT203520.1841407011569776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA203190.18384212395875726Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC195680.17704723075077328Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT195300.1767034145831256TruSeq Adapter, Index 1 (97% over 35bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC191240.17303001026562695Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC191150.17294858012065775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC186120.16839753979626904Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC183020.16559272369177494Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC177650.16073405837528043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA177410.16051691132202925Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT176950.16010071280329788Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC168180.15216579756574536No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC167140.15122482700165701Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA165960.15015718732317218No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG163840.14823905501945364Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA162870.14736141901256358No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG162770.14727094107370892No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC160500.14521709186170845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA159400.1442218345343073No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT156070.1412089191704476No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC155680.14085605520891448Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG154870.14012318390419182No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC153080.13850362879869366No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC152300.1377979008756274Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG151480.13705598177701928TruSeq Adapter, Index 15 (97% over 36bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG147950.13386211053545025No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG146100.13218826866663927Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA145510.13165444882739685Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC144580.13081300399604864No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG140950.12752865481562495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC138080.12493193797049659Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC136800.12377382035315711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA136390.12340286080385306No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC133230.12054375793604621Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA132850.12019994176839856Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC131290.11878848592226607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC129050.11676178009192198No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC128270.11605605216885573Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG126300.11427363677341917Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT126010.1140112507507407Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG125480.11353171767481107Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC125290.11335980959098725Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA124830.11294361107225587No Hit
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC123080.11136024714229954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA122340.11069071039477515Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAAAGATCGGAAGAGCACACGTCTGAACTCC121830.11022927290661647Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA121470.10990355232673972Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA113140.10236674002014763No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG111390.10078337609019129No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC111190.100602420212482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)