FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002433196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6333772
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3537965.585865736878435Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1697382.6798880667002223TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1098601.734511441207546Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC987961.559828803436562Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA643211.015524398415352TruSeq Adapter, Index 12 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTC628450.9922207493417825TruSeq Adapter, Index 12 (100% over 50bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG614650.970432784760803No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT552090.8716606786603622TruSeq Adapter, Index 12 (100% over 38bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC517230.8166223855231922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG496300.7835773059087066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA471180.7439168950192713No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG445480.7033407580822296TruSeq Adapter, Index 12 (100% over 49bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA366110.578028384981335Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG300360.47421978561905925No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC292160.4612733139115206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC291780.4606733554665372No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT290700.4589682104123735TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT284010.44840578410463783Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG278300.43939061904975424TruSeq Adapter, Index 12 (100% over 37bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC260320.4110031115739562Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC233380.3684692155006527No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA227330.3589172455213102Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC210110.33172965493547923No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT209530.3308139288878728Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT204880.3234723321268906No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC190380.3005791809367309Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC189000.298400384478633Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT181180.2860538712160779TruSeq Adapter, Index 12 (100% over 38bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG177150.2796911540232266No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC170590.2693339766571957No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC169510.26762883160303214No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC163900.25877155034946003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC163830.258661031688542Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC157530.24871435220592092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA154910.24457779661156098No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT146600.23145765272257987Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT145810.23021037069221942TruSeq Adapter, Index 1 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC125930.19882307099150395No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG123630.1951917435613407Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC119260.18829222144403052Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT113460.17913496096796663No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG111730.17640357120527864No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA110820.17496682861334448Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC109530.1729301275764268No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA108520.1713355011831812Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT106800.16861989980062433TruSeq Adapter, Index 12 (100% over 36bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA104530.1650359375108545TruSeq Adapter, Index 12 (100% over 40bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC103100.16277819915210084Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC102170.16130987979990438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG102090.16118357275885523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA98060.15482085556600397No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC97910.15458402986403677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG96140.1517894865808242No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG94730.1495633249823328No Hit
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC93570.14773187288712003Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT93490.14760556584607087TruSeq Adapter, Index 12 (100% over 36bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC92910.1466898397984645Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA91310.14416369897748135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT89440.14121127189295732No Hit
TGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGTCTGAACTCCA89270.14094286943072784Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC88280.13937981979774453Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC88040.13900089867459706Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC87680.13843251698987585No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA85030.13424859625512253No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG84730.1337749448511882No Hit
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT82890.1308698829070576Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC81570.1287858167297465No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC81310.12837531884633674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC81230.12824901180528758No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC81080.12801218610332044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC80780.12753853469938609Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG77590.12250204143755096No Hit
ACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG76570.1208916266641742TruSeq Adapter, Index 12 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA76470.12073374286286276No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC75590.11934436541132204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC73940.11673928268968317Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCAC73790.11650245698771602No Hit
CAGTCGGTCCTGAGAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGT72880.11506571439578185Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG71620.11307637849925763No Hit
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA71480.1128553411774216No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAGATCGG69870.11031341197630734No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC68620.10833986445991425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGACGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG67630.10677681482693094TruSeq Adapter, Index 12 (100% over 37bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG67560.10666629616601292TruSeq Adapter, Index 12 (100% over 37bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA66410.10485063245093129Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGA65540.10347704337952171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC64690.10213503106837442Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGACGAACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT63360.10003517651093219TruSeq Adapter, Index 12 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)