FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7468125
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4822916.457993137501046Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1689452.262214411247803Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1470691.9692894802912377No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTA1314721.760441877981421TruSeq Adapter, Index 4 (97% over 38bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT1121041.5010996736128546TruSeq Adapter, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1023791.3708795715122604Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC968221.2964699974893297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC948381.2699037576366223Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA888521.189749769855218No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC705480.9446547828270149No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTATGCCG702330.940436856640723TruSeq Adapter, Index 4 (97% over 34bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC696540.9326839066030631Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG620520.8308912879738891Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG580830.777745418026613No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGA531560.7117716963762658TruSeq Adapter, Index 4 (97% over 38bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA471220.6309749769855217Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC364000.4874048037492677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT351930.47124278182274665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA336710.45086283370993385Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC327000.4378609088626664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT320420.4290501297179681Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC317820.4255686668340447Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC302620.40521549920495437Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAC264210.35378358021591766TruSeq Adapter, Index 4 (97% over 37bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACC255750.34245543560130554TruSeq Adapter, Index 4 (97% over 38bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC249280.33379194911708093Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACC249110.33356431500543976TruSeq Adapter, Index 4 (97% over 38bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG237220.31764331743242114No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG224780.3009858565570341Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT221050.2959912963427902Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC208140.27870449409992465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGA204970.27445978742991045TruSeq Adapter, Index 4 (97% over 38bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC204000.27316093396936986No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC198070.26522052054565237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC196860.2636003012804419Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT185790.2487773035400452TruSeq Adapter, Index 1 (97% over 35bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT173900.23285630596702653Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC172030.23035233073897396Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA167680.22452757552933303Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT164350.22006862498953886TruSeq Adapter, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA158380.2120746505983764Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC158100.2116997238262616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA151350.20266131056992215No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA141540.18952548330404217Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC141170.18903004435517617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC135180.18100928948029124Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC134540.18015231400117163Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT128790.17245292493095657Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC123830.16581136496777973No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC120580.16145953636287555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC120100.16081680475353585Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG119490.16Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA114930.1538940497112729TruSeq Adapter, Index 3 (100% over 36bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC114890.15384048874382794No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC113070.1514034647250816No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC111610.14944848941334002No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT110740.14828353837141184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC109470.1465829776550339Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA108590.14540463637124446No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA107530.14398527073395262No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC107050.14334253912461292No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT105490.14125366139425893Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG101850.13637961335676627Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA100260.1342505649008285No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC98700.13216168717047452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG96080.12865344380282867No Hit
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG95720.1281713950958239Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC95060.12728763913298186Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGA94790.12692610260272827TruSeq Adapter, Index 4 (97% over 38bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC93150.1247301029374843Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG93040.12458281027701062Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC92830.12430161519792451Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC90630.12135576198845092Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG90470.12114151811867102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC89590.11996317683488159Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC89110.11932044522554189Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA88530.11854381119758975Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG87910.11771361620219266No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA86370.11565151895556114TruSeq Adapter, Index 3 (100% over 36bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC85690.11474098250899656Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA84980.11379027533684827Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC83710.11208971462047033No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC82880.11097832454598712No Hit
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA81630.10930454431333167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC81510.10914386141099675Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCT80820.10821993472257092TruSeq Adapter, Index 4 (97% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA80080.1072290568248389No Hit
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT79690.10670683739225041TruSeq Adapter, Index 1 (97% over 35bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC79310.10619800820152313Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT78530.10515356933634615Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA78340.10489915474098252No Hit
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC78170.10467152062934137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACA77940.10436354506653275TruSeq Adapter, Index 4 (97% over 38bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG77300.10350656958741317No Hit
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA77210.10338605741066198Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT77000.10310486233157587No Hit
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC76370.10226127709431752Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)