FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5925631
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3944376.656455658477553Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1476232.4912621120012366No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1388302.3428728518532456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1090851.8409009943413621Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG898181.5157541871912037TruSeq Adapter, Index 2 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTA888821.4999584010546725TruSeq Adapter, Index 13 (97% over 38bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC819871.38359948501687No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC740571.2497740746934798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA735451.2411336446700783No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC645071.0886098037491703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTATGCCG521450.879990671035709TruSeq Adapter, Index 13 (97% over 34bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC477670.8061082439996685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG468470.7905824713013685Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG466890.7879160885988344No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACC390130.6583771416073664TruSeq Adapter, Index 13 (97% over 38bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA368670.6221615892045927Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA353370.5963415541737243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC284770.48057329253205266Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT282390.47655684263836207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT273080.4608454357012781Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC272110.4592084792320008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC254050.4287307123916424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC229690.3876211664209263Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGA217060.36630698064054273TruSeq Adapter, Index 13 (97% over 38bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG216990.36618884976131655No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC216760.36580070544385906Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTG205160.3462247311720895TruSeq Adapter, Index 25 (97% over 37bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC185610.31323246418820205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGA176800.29836484924559087TruSeq Adapter, Index 13 (97% over 38bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT171580.2895556608232946Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACC154840.2613055048483444TruSeq Adapter, Index 13 (97% over 38bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG152490.25733968247432215TruSeq Adapter, Index 2 (97% over 35bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG141740.23919815459315638Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG140640.2373418122053162TruSeq Adapter, Index 2 (97% over 35bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA140060.23636301349172772Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC131960.22269358318126797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC128960.217630831214431No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG127930.215892619705817Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC121780.20551397817380126No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC119350.2014131490806633Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC118100.19930366909448125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC116780.19707605822907298Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA114200.1927220915375932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC109930.18551610790479528Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA108860.18371039303662345Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC108210.18261346344380877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA106890.1803858525784005No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA106260.17932267466536475Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC104640.1765887886032728Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC103200.17415866765919105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA94480.159442935275585Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT90670.15301324027770208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC89410.15088688445163054No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA88940.15009371997682608No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC85980.14509847136954696No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA85940.14503096800998913No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC83680.14121702819497198No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT82930.13995134020326275TruSeq Adapter, Index 8 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA82350.13897254148967428No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT81960.13831438373398547Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC80600.13601926950901938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC75890.1280707489210854Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC73500.12403742318750526No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG72060.12160730224342353Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG71080.11995346993425679No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT71000.11981846321514114TruSeq Adapter, Index 8 (97% over 36bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC68920.11630828851813418Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA68900.11627453683835529No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC67760.11435069109095722Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC67190.11338876821725821Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC67050.11315250645880581Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC66530.1122749627845541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC66200.11171806006820201No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA65410.11038486871693495No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC65310.11021611031804038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA64860.10945669752301486Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG64480.1088154156072155TruSeq Adapter, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA63660.10743159673628007Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACC62970.10626716378390756TruSeq Adapter, Index 13 (97% over 38bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC62520.10550775098888204Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCT62060.10473146235396703TruSeq Adapter, Index 13 (97% over 38bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC61930.10451207643540408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT60810.10262198236778497No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG59990.10123816349684953No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG59970.10120441181707063Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT59270.10002310302480867Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)