FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9564379
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA102113110.676396240675949Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2409592.5193376381258Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1918332.0057026180162874TruSeq Adapter, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1180301.2340581651981797No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1178451.2321239047511605No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC992981.03820645334109Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG889270.9297728582273873TruSeq Adapter, Index 7 (97% over 36bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC826980.8646457862031607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC765480.8003446956671206No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCCGTC714650.7471995829525367RNA PCR Primer, Index 23 (100% over 34bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG660760.6908550989039643TruSeq Adapter, Index 7 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT652750.6824802739414655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA597030.6242224403696256TruSeq Adapter, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG589490.6163390221152883Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA428670.44819428422901264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC382000.3993986436547527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC377970.39518509251881384No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC363140.37967964255703374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG324790.33958294626342184TruSeq Adapter, Index 7 (97% over 36bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC317210.3316577061615814No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG308940.3230110391903123No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT297060.310589950481887Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA294400.3078087976229298TruSeq Adapter, Index 7 (97% over 35bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA285000.2979806634596977Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC274500.28700242849012986Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGT248370.2596823066087197TruSeq Adapter, Index 7 (97% over 36bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC248340.2596509402230924Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC229660.24012013743913746Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA224910.2351537930481425Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC217590.22750039495507235No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT211560.22119575144397768Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG198990.20805323586612365No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG195790.2047074880658744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG195010.2038919620395637No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGA192710.20148720580813453TruSeq Adapter, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC188970.19757686306659325Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA183990.19237004305245536TruSeq Adapter, Index 7 (97% over 35bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG176710.18475846680688834Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG174800.18276147358861458No Hit
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC173070.18095267868410483Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC169490.177209623332576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA167850.17549492758494828No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC165290.1728183293447489No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA157740.16492445562853586No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA157630.16480944554790228Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT156650.16378481028407596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA156310.16342932458029946No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA150690.15755335500611173No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC142670.1491680745817371No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC139090.14542501923020826Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC138590.1449022461364193Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC134630.14076188323361088Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT128580.13443632879876466No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA127850.13367308008183282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT125150.1308501053753725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC123840.1294804398696455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA123480.12910404324211744No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT123460.1290831323183659No Hit
CGCGACCTCAGATCAGAAGGTCGGAAGAGCACACGTCTGAACTCCAGTCA121210.12673065339631565Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTTGCTGATGCCATCACCG115810.12108470398339505No Hit
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA115700.1209696939027615TruSeq Adapter, Index 7 (97% over 35bp)
CGACTCTTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTG115140.12038418803771787TruSeq Adapter, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC115030.1202691779570843No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG112420.11754030240750601TruSeq Adapter, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC111900.11699661838996551No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC110380.11540738818484712No Hit
CGCGACCTCAGATCAGAAGATCGGGAGAGCACACGTCTGAACTCCAGTCA109250.1142259209928841Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA106110.11094290596388955No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAGCTCCAGTCA102660.10733577161674585Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CGCGACCTCAGATCAGAAGATCGGAGGAGCACACGTCTGAACTCCAGTCA102040.10668753298044756Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG101710.10634250273854684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG101390.10600792795852193TruSeq Adapter, Index 7 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT101290.10590337333976414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG101280.10589291787788835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT100570.10515058008470805Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA99990.1045441632959129Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA98350.10282946754828515No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG98140.10260990284889379No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG97930.10239033814950244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG97850.1023066944544962No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA97050.1014702575044339No Hit
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC96150.10052926593561382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)