FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14255646
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA166123411.653165349364034Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC5122933.593614768492427Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA3241822.274060396842065No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC2887692.0256465403251456TruSeq Adapter, Index 5 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA2172931.524259230342841TruSeq Adapter, Index 6 (97% over 36bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCG1434501.006267972703587TruSeq Adapter, Index 6 (96% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1087490.7628486285363708No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC1032240.7240920544744167TruSeq Adapter, Index 6 (97% over 36bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA1009990.7084842033815936TruSeq Adapter, Index 6 (97% over 36bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG932370.6540356010523831Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG790870.5547766828665639No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC753760.5287448916730957TruSeq Adapter, Index 5 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC652390.45763622357064704No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC643290.45125278784279577Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT622560.4367111809594599Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT574240.4028158387210232Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC520620.36520267127845346TruSeq Adapter, Index 5 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA486670.34138754567839297No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC486420.3412121765649905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA465050.32622162475134414No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA461170.3234998961113372Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC451580.31677273692121705No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT442670.31052258171955166Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG423600.29714542574920844No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT399300.2800995479264847Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC398890.2798119425805046No Hit
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC397010.27849316684771774Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG391980.27496474028605933TruSeq Adapter, Index 6 (97% over 36bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC387310.27168884524770043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC386940.27142929895986473Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC385780.2706155862736771TruSeq Adapter, Index 6 (97% over 36bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC361490.2535767232154895No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC359270.2520194454884752No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT343780.24115357522205585Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC338870.23770932583483065Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC327580.2297896566735734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA324530.22765015349006285Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC320170.2245917161523231No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG310130.2175488925580784No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC293920.20617795924505983Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA266490.1869364601225367Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA253740.17799263533900886TruSeq Adapter, Index 6 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA249730.17517971476003263No Hit
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG238620.1673863113604252TruSeq Adapter, Index 6 (97% over 36bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT233520.16380878144701405No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC227550.15962096701896217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG226170.15865292951298032No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG218780.15346901852080222No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC216250.15169428309316885No Hit
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC213540.14979328190388566Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG212760.14924613027006983No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG209400.14688916938594013No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC206730.14501622725480134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC204300.14331163947252898TruSeq Adapter, Index 6 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA201470.1413264611088126No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA194000.1360864320003457No Hit
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC190120.1333647033603388TruSeq Adapter, Index 5 (97% over 35bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG188740.13239666585435692No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG184380.1293382285166172Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA183160.128482427243213No Hit
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA180770.126805898519085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT177180.124287598050625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG176110.12353701824526227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC175680.12323538337020996Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC170450.11956666151782949Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA168010.11785505897102104No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA166510.11680284429060599Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC165520.11610838260153206TruSeq Adapter, Index 6 (97% over 36bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG158770.11137341653966436No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC158000.11083327967038464TruSeq Adapter, Index 6 (97% over 36bp)
GTCTGAGCGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC157910.11077014678955972TruSeq Adapter, Index 5 (97% over 35bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC154390.10830094967285242No Hit
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA154280.10822378726295533Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC153350.10757141416109799Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA152590.10703829205635437TruSeq Adapter, Index 6 (97% over 36bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA150860.10582473779160902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAGATCGGAAGAGCACACG146060.10245765081428088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)