FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10657487
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9936469.323454957064456Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC3333623.127960653388552Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA1776941.6673161318423377TruSeq Adapter, Index 1 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1725201.6187681017110318No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1687101.5830185859011605TruSeq Adapter, Index 2 (97% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1552781.4569851222900858No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1540941.44587556147148No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCCG1228071.1523072934548266TruSeq Adapter, Index 1 (96% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC821170.7705099710654115Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATA781790.7335594216535286TruSeq Adapter, Index 1 (97% over 36bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG686780.644410825929227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC625860.5872491329334955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA601780.5646546882956555TruSeq Adapter, Index 1 (97% over 36bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA574520.5390764258028182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC497120.4664514251811895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG494090.4636083534514281TruSeq Adapter, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT482490.45272398643319944Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC451890.4240117768851137No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC413150.3876617442742365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC410310.3849969509697736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG387780.3638568829593693No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC374700.351583820838815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC353830.3320013432810192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC329910.30955702784343064No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT327380.3071831098644549Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA295710.2774669112896877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG290980.2730287168072549No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC283690.26618845512079914No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG275970.2589447212086677TruSeq Adapter, Index 2 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG274660.2577155383816091No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC252900.2372979671474148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC248790.2334415233159562Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA244600.2295100148843719No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC237850.22317643924876474No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC232670.21831600639062473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC231290.21702114203845613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA230530.21630802833726187No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA229240.21509761166023472No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA226560.21258294755602328Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT220180.20659654569599759Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC219430.205892815069819Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC216770.20339691711563898No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG210880.19787028593138326TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA200030.18768964953933323Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC198360.18612267601170895Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG188680.17703985939649752No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA188150.17654255642066463No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA181210.17003070235975892No Hit
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA175420.16459790192566034Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC175110.16430702660017318No Hit
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC172880.1622146008716689Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC169260.15881792771598033No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA168930.15850828624046176TruSeq Adapter, Index 1 (97% over 36bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA167050.15674426813750747Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT165560.15534618996016603No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC163180.15311301810642602No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC161350.1513959153785503Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG159600.1497538772508003No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG154140.1446307182922203No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCG152190.142801018664156No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA151610.14225680031324459Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC141410.13268606379721598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA141000.1323013577215717Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA139520.13091266261924597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT134080.1258082698106974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG132980.12477613155896883Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG129850.12183922907905026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG128030.12013150942619025No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA127980.12008459405111167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA124300.11663162244532882No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGA122790.11521477811795594No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG121990.1144641321166988Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG121620.11411695834111737No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG117110.10988519150903023Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG116540.10935035623313451No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG115800.10865600868197164No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG115250.10813993955610737Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA113650.10663864755359309No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATA108850.10213477154605022TruSeq Adapter, Index 1 (97% over 36bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC108130.10145919014491879Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC107350.10072731029369306No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC107100.10049273341830021Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)