FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569812
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1218073.412140471262912Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG848182.3759794633442883No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC500001.4006339829660497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC384711.077675799173738No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC376331.0542011736192272Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC332050.9301610280877537Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG320220.8970220280507769TruSeq Adapter, Index 8 (100% over 49bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC307500.8613898995241206Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG287680.8058687684393464No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC275740.7724216289261171TruSeq Adapter, Index 5 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC268950.7534010194374382Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG252680.7078243896317229Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA252280.7067038824453501No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC232650.6517149922741029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA209970.5881822348067629No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA199170.5579285407746962Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG187200.5243973632224891TruSeq Adapter, Index 8 (100% over 38bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA171580.4806415575946296TruSeq Adapter, Index 8 (100% over 40bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT155880.4366616505294956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC148110.4148957984342032No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC136150.38139263356165537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT135760.3803001390549418Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG132600.3714481322825964No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA119550.3348915853271825TruSeq Adapter, Index 8 (100% over 40bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC118490.33192224128329445Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT110520.30959613559481564Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG108190.30306918123419385Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG99430.2785300738526287TruSeq Adapter, Index 8 (100% over 38bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC97060.27189106877336955Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA95990.2688937120498222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC94210.2639074550704631Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC93420.26169445337737673Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC92940.26034984475372935Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT91540.2564280696014244No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA89940.25194604085593303Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT89870.2517499520983178No Hit
CTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA89190.24984508988148396TruSeq Adapter, Index 8 (100% over 39bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC79570.22289689204921717Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA78680.2204037635595376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG78330.21942331977146137TruSeq Adapter, Index 8 (100% over 38bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT78230.21914319297486815TruSeq Adapter, Index 8 (100% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC77190.21622987429029877TruSeq Adapter, Index 5 (100% over 35bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG76600.21457712619039884No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC74520.20875048882126007No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC74390.20838632398568888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC71750.20099097655562814No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG70890.19858188610492652No Hit
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT68380.19155070351043696TruSeq Adapter, Index 8 (100% over 37bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA68300.1913266020731624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT67130.18804911855302184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTAGA66950.18754489031915406No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC63700.17844076942987475No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC61190.17140958683538515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT60490.1694486992592327Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA60480.16942068657957338Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC60420.16925261050161747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC60220.16869235690843104No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC60200.1686363315491124Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG59940.16790800187797003No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC58550.16401423940532442No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA55420.15524627067195695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC53950.1511284067620368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT53700.15042808977055375TruSeq Adapter, Index 8 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA52900.14818707539780807Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC52750.14776688520291825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC50950.14272460286424046No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC50840.14241646338798794No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC49200.1378223839238593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA48600.13614162314430003No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC47710.13364849465462048Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACA47060.1318276704767646No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACG45800.12829807283969016No Hit
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC45760.12818602212105287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC45750.12815800944139355TruSeq Adapter, Index 5 (100% over 35bp)
ACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTAGA45640.12784986996514103No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG44540.1247684752026157No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC44450.12451636108568181Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC44430.12446033572636318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG43590.12210727063498021TruSeq Adapter, Index 8 (100% over 45bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC43390.1215470170417938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT43340.12140695364349718Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC42700.11961414214530065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC42470.11896985051313626Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG42160.11810145744369732No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCAC41900.11737312777255497No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC41400.11597249378958892Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA41270.11560832895401775No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA40260.11277904830842632No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG40140.11244289615251447Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG40020.11210674399660261Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG38950.10910938727305527No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC38450.10770875329008922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC37960.1063361319867825No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT36980.10359088938016903TruSeq Adapter, Index 8 (100% over 36bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC36820.1031426865056199Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG36540.10235833147515891Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC36510.10227429343618095Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA36140.10123782428878608Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTAAGATCGGAAGA36130.10120981160912676No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG36050.10098571017185219Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGA35930.10064955801594035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)