FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798648
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1558193.2471437788310373No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1412572.9436833041306634Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC735371.532452474113542No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG724981.5108005421527064TruSeq Adapter, Index 9 (100% over 49bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG524101.0921826314411893No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA479610.9994690171064851TruSeq Adapter, Index 7 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC393840.8207311726136195TruSeq Adapter, Index 9 (100% over 50bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC387220.8069356201996897Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC377820.7873467693400308Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG368760.7684664513838064No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA334370.6968004321217143TruSeq Adapter, Index 9 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA327700.6829006836925734No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC320590.6680840103295761Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC263680.5494881058164716Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA237440.49480603703376447TruSeq Adapter, Index 9 (100% over 38bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC224160.46713157539373595No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC210470.4386027064289775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG200700.41824280505675765Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC188340.3924855500966105No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA187800.39136023313233226Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA175960.36668661673037906TruSeq Adapter, Index 9 (100% over 38bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC167810.34970266625099405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT166460.34688937384029833Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA147460.30729488806013694No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG142570.29710451777250596No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA137270.2860597401601451No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG122780.2558637349520115No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC120310.25071645180059055No Hit
CGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG112320.2340659285698805TruSeq Adapter, Index 9 (100% over 45bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCATTTCTTAGCAGGGC111620.2326071843569272No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC105930.22074967782592098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC104480.2177279933848034TruSeq Adapter, Index 9 (100% over 37bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA104310.21737372693308615TruSeq Adapter, Index 9 (100% over 40bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG99930.20824615600060684Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC97320.20280712400659517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATC96940.20201523429099194TruSeq Adapter, Index 9 (100% over 42bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC96340.20076488210846055Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA95530.19907690666204314No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC95110.19820166013427115Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTAGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA94460.1968471119365288TruSeq Adapter, Index 9 (100% over 40bp)
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA87950.18328079075606296TruSeq Adapter, Index 9 (100% over 38bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC82920.17279867162584128No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT82170.17123573139767703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC80920.16863083101740323No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC80210.16715124760140773No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG77950.16244158771387274No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG74350.15493947461868426No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT71900.14983386987334765No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC69960.14579106448316276Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC68510.14276938004204517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT67230.14010196205264483TruSeq Adapter, Index 9 (100% over 36bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC66340.13824727298188988No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA61930.12905718444028402TruSeq Adapter, Index 7 (97% over 35bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA61620.1284111691459761Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA60920.1269524249330228No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT58190.12126332250250488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG57960.1207840208325345No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA57960.1207840208325345TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA56860.11849170849789357No Hit
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG56420.1175747835640372TruSeq Adapter, Index 9 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT56120.1169496074727715No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC55760.11619939616325264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA55690.11605352174195731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC55220.11507407919897436No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA54700.1139904406407805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA53460.1114063794635489No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG53210.11088539938749414Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT52810.11005183126580655No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC52500.10940581597149865Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC52280.10894735350457044No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT52120.1086139262558954TruSeq Adapter, Index 9 (100% over 36bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT51340.10698846841860458Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC50140.10448776405354175Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC49680.10352916071360099Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTAAAAGTCAGATC49390.10292482382537747No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG49390.10292482382537747No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC49260.102653914185829No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA48850.10179950686109922No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA48400.10086174272420066Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)