FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7727500
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA156756320.285512779035912Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC3185504.1222905208670335Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT2049902.6527337431252023TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1177611.5239210611452605No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA687010.8890456163054028TruSeq Adapter, Index 3 (100% over 40bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC575780.745105143966354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC560170.7249045616305403Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG542200.7016499514720155TruSeq Adapter, Index 3 (100% over 38bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG469100.6070527337431252Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG436300.5646069233257845No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG413440.5350242639922356TruSeq Adapter, Index 3 (100% over 37bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT394440.5104367518602394Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC384840.49801358783565186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC383010.4956454221934649No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC367960.4761695244257522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA303190.39235198964736334Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG302330.391239081203494TruSeq Adapter, Index 3 (100% over 49bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC302040.39086379812358457Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG267510.3461792300226464TruSeq Adapter, Index 3 (100% over 38bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT220430.2852539631187318TruSeq Adapter, Index 1 (97% over 35bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT219000.2834034293109026TruSeq Adapter, Index 1 (97% over 35bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA217580.2815658362989324TruSeq Adapter, Index 3 (100% over 36bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC201400.26062762859915883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA198970.25748301520543515No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC197240.2552442575218376No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA190960.24711743772241995No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT189720.24551277903591073Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT184150.23830475574247817Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC174380.22566159818828857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA171680.22216758330637332TruSeq Adapter, Index 3 (100% over 40bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT170510.22065351019087673Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC166510.21547719184729863Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA148320.19193788417987706Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG143470.1856615981882886No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC142980.18502749919120026Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC136310.1763959883532837No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA133570.1728502102879327Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA132870.17194435457780652No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC132690.1717114202523455No Hit
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA130100.1683597541248787Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA123040.1592235522484633Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT122280.15824005176318345Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC121600.15736007764477514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC118160.15290844386929794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC115260.1491556130702038No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG108190.14000647039792946Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC105710.13679715302491105No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA103880.13442898738272405No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC98940.12803623422840504No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC95170.12315755418958266No Hit
CACCCGTAGAACCGACCTTGCGAGATCGGAAGAGCACACGTCTGAACTCC94880.12278227110967324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA90350.11692009058557101Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG90120.11662245228081526Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT84810.10975088967971532Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA84690.10959560012940796No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTC84380.10919443545778067TruSeq Adapter, Index 3 (100% over 50bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG84310.10910384988676804No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC83680.10828857974765449Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC83540.10810740860562926Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA82370.10659333549013264Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC79120.10238757683597541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG78790.10196053057263021No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT77900.10080879974118408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)