FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5153160
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4029107.818697653478643Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1367912.6545071373681393Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC777441.5086665269465727TruSeq Adapter, Index 5 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA696621.3518307213437968No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG594621.1538939213996848TruSeq Adapter, Index 8 (100% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG491140.9530850972995211No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA490210.9512803794176776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC412090.7996840773428344Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC353550.6860838786298116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC352020.6831148266306499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA334010.6481653975424788TruSeq Adapter, Index 8 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTC278370.5401928137298279TruSeq Adapter, Index 8 (100% over 50bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC275570.5347592545156759No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG271300.5264730767140939Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG224090.4348593872497652TruSeq Adapter, Index 8 (100% over 38bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC182980.3550830946448393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC173580.3368418601401858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG164500.31922160383143544TruSeq Adapter, Index 8 (100% over 38bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT159460.3094411972459617Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC158640.30784994061896004TruSeq Adapter, Index 5 (100% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT156890.304453966110115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG156650.3039882324631876No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC155650.30204767560099044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC150730.2925001358389803TruSeq Adapter, Index 5 (100% over 35bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC147470.28617392046821755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA138840.26942691474745595Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT132290.2567162673000644Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT129870.25202011969354726TruSeq Adapter, Index 8 (100% over 37bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC124500.2415993293435484Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA123670.23998866714792477TruSeq Adapter, Index 8 (100% over 40bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA120700.23422521326719914Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC120630.23408937428684534No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA118070.22912154871962057No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA117060.22716158628880143No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG114300.2218056493491372No Hit
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC111700.2167602015074246Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC105870.20544675500081505No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA104730.20323452017791027Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC103990.20179850809988434Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG99310.19271670198480156Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC96250.1867785979864782No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC91970.1784730146162743Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA91500.17756095289104162No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC89280.17325291665696388Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC88110.17098246512819318Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC87660.17010921454020445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC80050.15534157681888394No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA78540.1524113359569662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC76870.14917060599709694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT73410.1424562792538947TruSeq Adapter, Index 8 (100% over 36bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA70800.13739142584356007Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC70100.13603303604002204No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA69400.134674646236484No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA66540.1291246536106001No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT65470.12704825776804912No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG64560.1252823510234497Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC63180.1226043825536176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC63070.12239092129877589No Hit
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG61570.11948008600548013No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC61510.11936365259374832No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC58250.1130374372229855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58120.11278516483089987Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG57440.11146558616460579Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT57410.11140736945873989Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC56520.10968027385138439No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG56480.1096026515768965No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG55300.10731279447950383No Hit
CACGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA54730.10620667706805144TruSeq Adapter, Index 8 (100% over 40bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC54590.10593499910734384TruSeq Adapter, Index 5 (100% over 35bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA52780.10242259118676696TruSeq Adapter, Index 8 (100% over 40bp)
CACGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC52400.10168517957913202TruSeq Adapter, Index 5 (100% over 35bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC52040.10098657910874104No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTATTTAGCAGGG51860.10063727887354557No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)