FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4613613
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2061444.468168439788946Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1959284.246736776578356No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1215452.6344862475461204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC778201.6867474580117578No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC751661.6292220435480824Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC598661.2975947484108443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC511631.1089573399416033Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA466641.011441575181967Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC421040.9126036362391038Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG412480.8940498477007066No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG369310.8004789305041407No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA368630.799005031414642No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC349740.7580609817078285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC306660.6646851393907551Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA272120.5898197356388583Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG261400.5665841499926413TruSeq Adapter, Index 21 (97% over 49bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC240390.5210450031244493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG236260.512093233654405Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA234440.5081483860913345Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT217290.4709757840547094TruSeq Adapter, Index 1 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT215130.46629398694688956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC211290.4579707920885432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC203660.4414327773049018Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATCG151670.32874452191807163No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC145990.3164331295234342No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT145510.31539273016614094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG141040.3057040111513471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC134380.29126847006890266Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC129400.28047432673698464No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC126100.27332158115559324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA125160.2712841324142272TruSeq Adapter, Index 21 (97% over 39bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT122940.2664722853867457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT116610.25275201886244036Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC116020.251473194652434No Hit
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA114840.2489155462324213Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG106310.23042678265385502Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG105410.2284760338589301Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC101600.2202178639604145TruSeq Adapter, Index 21 (100% over 38bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC101140.2192208145763418Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC100470.21776859047345326No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC97810.21200304403511955TruSeq Adapter, Index 21 (100% over 38bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC92650.2008187509442166Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT91770.19891135212251224Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC90740.1966788285016537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC86680.1878787839378812Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC84710.18360881157565664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA82040.1778215901507127TruSeq Adapter, Index 21 (97% over 39bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC80770.17506886685120748No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG79330.1719476687793276No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC77080.16707079679201528Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA76890.16665897204642002Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC76490.16579197258200895No Hit
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC72870.15794562742908866Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC72420.15697025303162618Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT71890.15582147874128152TruSeq Adapter, Index 1 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC71340.15462935447771625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA69510.15066283192803556No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA67020.1452657602620766No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA66810.1448105855432608No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC66740.14465886063698885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG65910.14285983674833586No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA65370.1416893874713809Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC63620.13789626481458242Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC62110.13462334183643057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC62050.1344932919167689Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT61980.13434156701049696TruSeq Adapter, Index 21 (100% over 37bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG61310.13288934290760843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG60460.13104696904573487No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG60340.13078686920641155No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT60280.13065681928674988TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC59750.1295080449964052Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATC59110.12812084585334746No Hit
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC58540.12688537161656166Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC58080.12588832223248894Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTATTTAGCAGGG57750.12517304767434978No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC55990.12135825003094104Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT55910.12118485013805882Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA54710.1185838517448256No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA54200.11747842742770147No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG53530.1160262033248129Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT53390.11572275351226902Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC52800.11444392930226267Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG52610.1140321045566674Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA52390.11355525485124132Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC51680.11201633080191165Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC51660.1119729808286911Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC51430.11147445613665471No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC51090.1107375065919053Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC50150.10870005785053925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCC48680.10551383481882855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC46880.10161233722897868No Hit
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC46460.10070198779134705Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)