FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053369
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5350427.585623267405974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1355761.9221452897189981Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1354581.9204723303147757Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1322371.874806209628335No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG1001761.42025746845231TruSeq Adapter, Index 14 (97% over 49bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC943481.3376302870302121Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG902081.278934931661735TruSeq Adapter, Index 2 (97% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC828501.1746159884730263No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA805101.141440352829974No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC741921.0518661365937327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC636050.9017676517420257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG601220.8523869940733286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTC595140.8437669998549628TruSeq Adapter, Index 14 (98% over 50bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG509890.722902771710937No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC465500.6599683073436254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA457890.6491791369485986TruSeq Adapter, Index 14 (97% over 39bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA411980.5840896740266956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT403290.5717693204481433Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA352660.49998801990935116Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC346940.49187842008549393Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT330460.46851369891466055Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC307240.43559326046886243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC305150.4326301374562993Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC286100.405621767413558TruSeq Adapter, Index 14 (100% over 38bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC271580.3850358601683819Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC263050.3729423485429445TruSeq Adapter, Index 14 (100% over 38bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT255930.362847881629332Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG236940.3359245773190088Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC221320.31377913164616794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG210990.29913364804818804No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC204120.28939362168631755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT195820.27762619536848276TruSeq Adapter, Index 8 (97% over 37bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG180490.25589190073566265No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA174850.24789572188836287TruSeq Adapter, Index 14 (97% over 39bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC168070.2382832941251195Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG162770.23076915442818885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA161940.22959241179640538No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA161290.22867086636187617Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG151340.21456413240254407TruSeq Adapter, Index 2 (97% over 35bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA149850.2124516667141617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC148660.21076452968786974Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC139530.19782036073825146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC134980.19136954269654685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG133420.18915783365367672TruSeq Adapter, Index 2 (97% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC133100.18870414974744693No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC126800.17977224784354826No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC124610.1766673486102882Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT124020.17583086890817706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT123940.17571744793161964Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC123500.17509363256055369No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC122900.17424297523637286Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT122290.17337814029012236TruSeq Adapter, Index 8 (97% over 36bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA118290.16770709146225019Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA117670.16682807889392998Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC113430.16081676713638546Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC110250.15630828331822708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC107640.1526079239580405Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA104930.14876578837715707Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCG104510.1481703282502305TruSeq Adapter, Index 14 (97% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC103570.14683763177568052No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC103420.14662496744463532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC101960.14455503462246197Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG97760.13860043335319616Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA95760.13576490893926008No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA95280.13508438307991544No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC94470.13393599569227133Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC91530.12976777480378524Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC91020.12904471607823156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT88800.1258972839787625TruSeq Adapter, Index 8 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC86580.12274985187929342No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT86040.12198426028753068No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC84180.1193472225825701Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA83370.11819883519492601No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC83350.11817047995078664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG80290.11383212759746443No Hit
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA79190.11227258916979956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA79050.11207410246082403Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA78920.11188979337391819Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA75470.10699851375987844Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGTGCATCCCTTTAGAGTGCAGATCGGAAGAGCACACGTCTGAACTCC75460.10698433613780876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC75320.10678584942883323No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT75190.1066015403419274Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC75020.10636052076674282Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC74950.10626127741225505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA73020.10352499635280672TruSeq Adapter, Index 14 (97% over 39bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC71330.10112897822303073Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)