FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8946601
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7121407.959894489538541Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2543742.8432473964134535Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1719121.921534222885317No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1372011.533554475045886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1225221.3694809906019056No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1183561.3229158202092617Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1091571.2200946482356818TruSeq Adapter, Index 2 (97% over 35bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA971641.086043738845624Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTC840920.939932383259296TruSeq Adapter, Index 14 (98% over 50bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC711360.7951176094697863Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG667000.7455345331707539TruSeq Adapter, Index 14 (97% over 49bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC664360.742583691840063No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC636780.7117563418777701Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC529160.5914648479349867Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG523930.5856190524200197Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG415060.46393037981687124No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA407660.4556590821475105Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA405840.4536247900180191TruSeq Adapter, Index 14 (97% over 39bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT385750.43116933458863316Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG381530.42645245943124094No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC379730.4244405221603154TruSeq Adapter, Index 14 (100% over 38bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC367380.41063639699590937Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG366430.409574541214032TruSeq Adapter, Index 2 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC355190.3970111107000301Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC351070.3924060098354671No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC281240.31435402115283784Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC279320.3122079547305172Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT252240.28193947623237026Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG244830.27365700113372665No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA243720.27241630648332255Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC235510.26323963704204534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC215810.24122010135469324Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG212730.23777745313555396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG209270.23391006260366368TruSeq Adapter, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA209100.23372004630585405No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT205640.2298526557739638TruSeq Adapter, Index 8 (97% over 37bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA196910.22009476000997472Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC193150.21589204659959688Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT192850.2155567237211093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC192610.2152884654183192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA174450.19499025384053675No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG166870.18651776244408352Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC166200.18576887468212788TruSeq Adapter, Index 14 (100% over 38bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC162790.1819573712966522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG162470.1815996935595988No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG160010.17885004595600051Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC157850.17643572123088982Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA155900.17425612252072042No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC152380.17032166741313265No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC152030.16993045738823045No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC151770.16963984422687456Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG149840.16748260037527102No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC148530.1660183571392085Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA148290.16575009883641842Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT143860.16079849766408494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC142510.15928954471089077Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC142500.15927836728160785Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA136030.1520465705355587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC133860.1496210683811651Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA133440.14915161635128246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA131960.1474973568174103No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC131790.14730734051960068Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA131180.1466255173333426Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC127290.14227749734228676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC127010.141964529322365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC123660.13822009051258685Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC122060.13643170182731967No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA121810.13615226609524667TruSeq Adapter, Index 14 (97% over 39bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC118360.13229605299263933No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG117140.13093240662012312Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC115710.12933403423266557No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC114850.12837277531433447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC112790.12607022488205297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC111770.12493012709519513Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC111770.12493012709519513Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG111190.12428183619678579Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC110710.12374531959120565No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC107100.11971026762007159Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG100420.11224374485908112No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA99970.11174076054134972Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT99590.11131601822859877TruSeq Adapter, Index 8 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT97830.10934879067480488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA94600.10573848101642176No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGA94450.10557081957717797No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG94320.10542551299650002No Hit
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA93600.10462073808812979Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)