FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7436798
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3642664.898156437757217Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1770582.380836483658693No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1230851.6550805871021372Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC998461.3425939497079253Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC997631.3414778779792056No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC900251.210534426240971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT882831.187110366585189TruSeq Adapter, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC857081.152485249700207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA786941.0581704652997164No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC781821.0512857818647219Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA614150.825825846015987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG587750.7903266970542968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG576910.7757505313442694No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC497930.6695489107005461Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG473230.6363356917856314TruSeq Adapter, Index 18 (100% over 49bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT454500.611150121329099Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCCGTC424230.5704471198491609TruSeq Adapter, Index 18 (100% over 50bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC394910.5310215498659504No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA389410.5236258938322649TruSeq Adapter, Index 18 (100% over 40bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC341280.4589071802138501Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA336090.45192837024751786Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG314860.4233811379574919No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC298170.40093868355709Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC293630.394833905667466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT286730.38555571900702423Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG271020.36443103604535176TruSeq Adapter, Index 18 (100% over 38bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC265080.35644372752897147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG260760.3506347758806949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC249490.3354804043353067No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG243750.32776202876560584TruSeq Adapter, Index 18 (100% over 38bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG211440.28431591122953725Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC187920.2526893967000314No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC184760.24844025614249574TruSeq Adapter, Index 18 (100% over 37bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC183960.24736452435577785No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC183890.24727039782444005Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC167320.22498930319204583Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC165310.22228652707791713Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT161290.21688097484965974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA153050.20580093744646555TruSeq Adapter, Index 18 (100% over 40bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT147560.19841872806011404TruSeq Adapter, Index 1 (97% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC147190.19792120210875702No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT139010.18692184458956665Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT138860.18672014487955704TruSeq Adapter, Index 1 (97% over 35bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA137950.18549649997216544No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC137150.18442076818544756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA134700.18112633958862404Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC133870.18001026785990423Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG133600.17964720838188694Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC131780.17719991856710374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA126810.17051693484211888Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG124480.16738386601330305Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG119650.1608891353509938Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT116140.15616936213676907Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC113140.152135367936577Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG109760.14759040113769395No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC109460.14718700171767474No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG109430.14714666177567282No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC108780.1462726296989645Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG108080.14533136438558636Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC107580.1446590320188877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC107040.14393291306285314Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC104420.14040989146135205Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC98000.131777143872941Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA97270.13079553861756094Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTC95490.12840203539211364TruSeq Adapter, Index 1 (97% over 36bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA94760.12742043013673357Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA94000.1263984849393516No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC93810.12614299864000608No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA92450.12431425460258567No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG92310.12412600153991005Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC91990.1236957088252229No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA90970.1223241507971576No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC90720.12198798461380825Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC88310.11874734260632062Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA88100.11846496301230719No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT87930.11823637000762963No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG87700.11792709711894823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC86850.11678413209556048Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC85830.11541257406749517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG85180.11453854199078689No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC83810.1126963513060325No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC82450.11086760726861211No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC82340.1107196941479384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC80390.10809759791781355Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC79860.10738492560911296Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC78200.10515278215167334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC77470.10417117689629327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC77350.1040098171282856Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT76900.10340471799825679Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT75750.10185835355484982No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC74740.10050024217411847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)