FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442915
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3963438.920787365952307Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1056642.3782584181781554Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT756331.7023283137309626TruSeq Adapter, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC563851.2690992287721012Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG520961.1725635084173343TruSeq Adapter, Index 21 (97% over 49bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG515861.1610845582235987No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC508121.1436635632236944Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA371910.8370855620690469No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA362100.8150054637552147TruSeq Adapter, Index 21 (97% over 39bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG353400.7954237251894308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC347470.7820766321210285TruSeq Adapter, Index 21 (100% over 38bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT328400.7391543614946493Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC285060.6416057925933762No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC231100.5201539979945599Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC226260.5092602491832502TruSeq Adapter, Index 21 (100% over 38bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA219430.4938874590218359Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC206150.463997173027168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC192470.43320657721338357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG179260.403473845437061No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT159000.3578731530988101TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT157600.3547220687319024TruSeq Adapter, Index 21 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC133840.3012436654763821No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA132830.2989703831831129TruSeq Adapter, Index 21 (97% over 39bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC132320.29782248816373935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC132150.29743985649061483Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC131590.29617942274385173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG130020.29264570670381945No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT128350.28888691320900806TruSeq Adapter, Index 1 (97% over 35bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG123950.2789835051987265Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC123690.27839830381630076No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA115780.26059467714327195No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT114950.2587265342686052Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC112360.25289702818982585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA109600.24668489043792194Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT103620.2332252586421302TruSeq Adapter, Index 9 (97% over 36bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC97380.21918042546391278Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG95490.2149264615685873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA91630.20623847181411303Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT91560.20608091759576766Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC86460.19460196740203223No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC85220.19181100696277106Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA83320.18753453532196768Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC79720.17943174694991915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT78770.17729351112951744TruSeq Adapter, Index 9 (97% over 36bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA77420.17425496548999925Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC74970.16874056784791067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG74130.166849917227766Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC71190.1602326400572597Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC68540.15426808750561288Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC68190.15348031641388593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA66540.14976653841003035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATC66020.1485961356451789TruSeq Adapter, Index 21 (97% over 42bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC65770.14803344200823107No Hit
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC65060.1464353920792993TruSeq Adapter, Index 21 (100% over 38bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG63840.14368944713099396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG63440.14278913731187745No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA62190.13997566912713838No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG59100.1330207757744634No Hit
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA58920.13261563635586096Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA55420.12473792543859157No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT54240.12208201147219787Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC53720.12091160870734642Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG52430.1180081095406957TruSeq Adapter, Index 21 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC52070.11719783070349082Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC51540.11600492019316147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC51160.1151496258650008No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC50900.11456442448257508Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC50150.11287634357173162No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC49990.11251621964408502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA49700.11186349502522556No Hit
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT49340.11105321618802069TruSeq Adapter, Index 21 (100% over 37bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG49050.11040049156916124Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT48940.11015290636890421TruSeq Adapter, Index 21 (100% over 37bp)
GTAGTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA46550.1047735551996831TruSeq Adapter, Index 21 (97% over 39bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC46450.10454847774490396Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA46120.10380572214413286Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC44840.10092473072296004Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC44760.10074466875913675No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)