FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7937864
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7301969.19889783951955Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1905332.4003056741712885Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT1376681.734320467067715TruSeq Adapter, Index 1 (97% over 35bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG952601.2000709510770151No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC938351.1821190184160373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC935221.178175892154363Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG887881.1185376821774724TruSeq Adapter, Index 21 (97% over 49bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC663850.8363081050519383TruSeq Adapter, Index 21 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA658130.8291021362925844No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA652720.8222867008051536TruSeq Adapter, Index 21 (97% over 39bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG630600.7944202621763236Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT591250.7448477323370619Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC534160.6729266210658182No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC439710.5539399516041091TruSeq Adapter, Index 21 (100% over 38bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC414430.5220925931711604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA393510.4957378962400968Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC361810.4558027197240971Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG345100.43475171658269784No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC338480.426411941550019Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT287750.362503061276938TruSeq Adapter, Index 21 (100% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT276880.3488092010646693TruSeq Adapter, Index 1 (97% over 35bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT250690.31581543851091426TruSeq Adapter, Index 1 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC249240.31398875062611303No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG240570.30306641685974967No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC237830.29961460664984935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC233080.2936306290961901Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC232960.2934794549264135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC230970.29097248327761727No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG225900.2845853746045536Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT224200.28244374053271765Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA202560.2551819985829941Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC198210.24970193492859036Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT188070.236927717582463TruSeq Adapter, Index 9 (97% over 36bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGA182730.23020046702740185TruSeq Adapter, Index 21 (97% over 39bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC174130.2193663181934082Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA170440.21471771247277605Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC166420.2096533777852581No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG163750.20628975250772752Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA163300.20572284937106505Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT162640.20489139143729346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC157630.1985798698491181Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT153180.19297382771990046TruSeq Adapter, Index 9 (97% over 36bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA148860.18753155760794088No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC142410.17940594598244564Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA142200.1791413911853365Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC139170.17532424339847597Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG133800.16855919930097063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC132880.16740019733268297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC130580.16450269241196372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC125910.15861949763815555TruSeq Adapter, Index 21 (100% over 38bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG120930.15234576959242435Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATC119870.15101039775939723TruSeq Adapter, Index 21 (97% over 42bp)
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA115600.1456311168848446Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC115160.14507681159566352No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG114160.1438170268475247No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA111660.14066756497717775No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA110680.13943297592400172Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC109460.13789603853127239Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG104660.1318490717402062TruSeq Adapter, Index 21 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG99320.125121821185145No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA97980.12343370962263904No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC97870.12329513330034376Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT95320.12008268219258986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT93510.11780247179845862TruSeq Adapter, Index 21 (100% over 37bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT92780.1168828289323173TruSeq Adapter, Index 21 (100% over 37bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG90600.11413649818137475Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC90390.11387194338426558Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC89540.1128011263483476No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA88310.11125159110813689No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA87340.11002959990244227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC86340.10876981515430348Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC82590.10404562234878299No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC82130.10346612136463915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT81090.10215594522657481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)