FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4018969
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2634616.555437476626468Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1210463.0118669738432917Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1148582.857897137300636No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC880962.1920049644573023Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC838112.085385580232144Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC735191.8293000020652064Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC550491.3697294007492966No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC494131.2294944300391468Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC459201.142581592443236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG428431.0660196682283443No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC397410.9888356939304583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA351030.873432962533426TruSeq Adapter, Index 3 (100% over 40bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT283220.7047080980221544TruSeq Adapter, Index 1 (97% over 35bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC256100.6372281050189738No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC255870.6366558189426194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC251900.6267776636246758Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG250380.6229955991200729Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC233000.5797506773503354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT231570.5761925508756102Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG223450.5559883641799676Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT221460.551036845519336Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC212520.5287923345514733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG203410.5061248295271747TruSeq Adapter, Index 3 (100% over 49bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA187010.46531834408277345No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC168510.41928663794122323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC157450.39176714226957216Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC153210.3812171728619952No Hit
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA151040.37581777814160794Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA141780.3527770430675131Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC138710.3451382680483477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA132580.3298856000133367Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC128610.3200074446953933Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG124360.30943259328449657Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG119600.2975887597042923TruSeq Adapter, Index 3 (100% over 38bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC117640.29271188705362Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC114740.2854961060908905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG113500.28241073767924063Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC112610.28019623938378224Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT106890.2659637334848813Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT102850.2559114041436995TruSeq Adapter, Index 1 (97% over 35bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC97090.2415793702315196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC94060.23404012322563325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC84230.20958111396231222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC82250.2046544773050004Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC80920.20134517086347273Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC79340.1974138143389511Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG79300.19731428632567208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC78120.19437820993394075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG77350.1924622956783195Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT77070.1917655995853663Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT76840.1911933135090119No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC72910.18141468620434745Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT72280.17984711999520275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG71600.17815514376945926No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG70580.17561717943084407TruSeq Adapter, Index 3 (100% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG69850.17380079318850183No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG68850.17131259285652614TruSeq Adapter, Index 3 (100% over 38bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG65440.16282782972448906Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCTCAGTTCAGCAGGAACAAGATCGGAAGAGCACACGTCTGAACTCCA61480.15297455640986532Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC61250.1524022703335109No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC59340.14764980769943734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC58500.14555971942057777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC57050.14195182893921302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC55790.13881669652092365Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA55730.13866740450100512No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC54780.13630361418562822Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT53110.1321483196312288TruSeq Adapter, Index 1 (97% over 35bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC52150.12975964731253214No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA50990.12687333492744035Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA50230.12498230267513882No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG49620.12346450047263365No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC49600.12341473646599414Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTGGAGGTAAGAAGCACTTTAAGATCGGAAGAGCACACGTCTGAACTC49270.12259363035644216Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGCTGTCATAGCTGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC49060.12207110828672726Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG47660.11858762782196132No Hit
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTCTGAACTCCAG47290.1176669936991303Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG46420.11550225941031145No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC46400.11545249540367193No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT45610.11348681714141115Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC45450.11308870508829504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA44030.10955546061688956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA43790.10895829253721538No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA42980.10694285026831508TruSeq Adapter, Index 3 (100% over 36bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCA42440.1055992220890482TruSeq Adapter, Index 3 (100% over 40bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC42390.10547481207244944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT41980.10445464993633939Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41230.10258849968735761Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA40840.1016181015578871Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA40270.10019982736866097TruSeq Adapter, Index 3 (100% over 36bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT40250.10015006336202145Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)