FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5409820
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3317216.131830633921276Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2215324.094997615447464No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1398702.5854834356780816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC961041.7764731543748222Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC907711.6778931646524282Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC882971.632161513691768No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA775931.434299107918563Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG669881.2382667075799194No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC666571.2321482045613348Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA506880.936962782495536No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA459680.8497140385447206TruSeq Adapter, Index 7 (100% over 35bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC373260.689967503539859Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC370600.685050519240936Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA366280.6770650409810308TruSeq Adapter, Index 7 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG350640.6481546520956335TruSeq Adapter, Index 7 (100% over 49bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC345560.6387643211788931Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG311510.575823225172002Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT303650.5612940911157858Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC271350.5015878532002913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC208230.3849111430694552Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA190380.3519155905372083Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA189180.34969740213167905TruSeq Adapter, Index 7 (100% over 35bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC187700.3469616364315264Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA184730.3414716201278416Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC184470.34099101263997694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC183730.33962312978990056Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT183070.3384031261668595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT177560.32821794440480456TruSeq Adapter, Index 7 (100% over 38bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC174240.32208095648284046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT153770.28424235926518815Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG139420.2577165229157347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG138160.25538742508992907No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG137560.25427833088716445Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA127350.23540524453678682No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT120910.22350096676044673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC120730.22316823849961734Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA116090.21459124333157112TruSeq Adapter, Index 7 (100% over 37bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC115300.2131309359645977Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC115080.2127242680902507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT112420.20780728379132762Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC112050.20712334236628946Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT107300.19834301326106968TruSeq Adapter, Index 7 (100% over 38bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA105750.1954778532372611Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC100510.18579176386645027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC100190.18520024695830917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC97580.18037568717628313No Hit
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC95580.17667870650040113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT92970.1718541467183751Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG90700.16765807365124902Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC88150.16294442328949946Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA87570.1618722988934937No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA87140.16107744804817906TruSeq Adapter, Index 7 (100% over 35bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA86450.15980198971499976No Hit
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG82830.15311045469165332No Hit
TCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGA81280.15024529466784478No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA81130.14996802111715363TruSeq Adapter, Index 7 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC81080.14987559660025657Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC81050.14982014189011836Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA78850.1457534631466481No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC78780.14562406882299225No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC74710.13810071314757238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC74020.1368252548143931Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC73600.13604888887245786Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC73190.13529100783390205No Hit
AAAGTGCTTCCCTTTGGACTGAGATCGGAAGAGCACACGTCTGAACTCCA72880.13471797582914033Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC72860.13468100602238153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC72830.1346255513122433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG72790.13455161169872565No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC72690.13436676266493155Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC70890.13103948005663774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA70170.12970856701332023No Hit
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA68420.12647370892192344No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG66900.12366400360825315Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC65710.12146430010610335Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG63730.11780428923698015Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC63180.11678761955111261Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC62830.11614064793283324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC62830.11614064793283324No Hit
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC62720.11593731399565975No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA62470.11547519141117449No Hit
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC62060.11471731037261867Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC61160.1130536690684718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA60710.11222184841639833Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC60210.11129760324742781No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT58540.10821062438306636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA58530.10819213947968695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG58470.10808123005941048No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG58380.1079148659289958Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58180.10754516786140758Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC57430.10615880010795184Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATAAGATCGGAAGAGCACACGTCTG57370.10604789068767537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA56490.1044212191902873No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC56410.10427333996325201No Hit
AAGTGCTGTCATAGCTGAGGTCAGATCGGAAGAGCACACGTCTGAACTCC56040.10358939853821385Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA55910.10334909479428152No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC55810.10316424576048741Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC55120.10188878742730813Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG54180.10015120650964358TruSeq Adapter, Index 7 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)