FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002433578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875855
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2555015.240127116167318Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1164782.38887333606106No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA797871.6363694162357167No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC776931.593423102204639Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG648511.3300436538822422TruSeq Adapter, Index 2 (97% over 35bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC620611.2728229202878265No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG564841.1584429807695265TruSeq Adapter, Index 22 (97% over 37bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG370940.7607691369000924No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG362960.7444027765386788Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC317570.6513114110243229No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA294910.6048375105494318TruSeq Adapter, Index 22 (97% over 37bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC288350.5913834599265154Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAG272650.5591839790149625TruSeq Adapter, Index 22 (97% over 37bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC258540.530245464641586Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC254890.5227595980602376Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC242840.498045983730033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTC232930.47772134323108456RNA PCR Primer, Index 24 (100% over 34bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA222830.45700702748543587No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT189790.3892445530066009Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC176460.3619057580670467No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTA175050.35901395755206006TruSeq Adapter, Index 22 (97% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG174700.3582961347291911No Hit
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA169180.3469750433513712TruSeq Adapter, Index 22 (97% over 37bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAG166970.3424425049555411TruSeq Adapter, Index 22 (97% over 37bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT164540.3374587636424791Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT163680.3356949704205724Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC141800.2908207893795037Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC134760.27638229602808123Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT123850.25400673317807854No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC122270.25076627586341266No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC121890.24998692537001205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG120520.2471771617490676Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG118710.24346499229365928No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT113950.23370260190264067No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA113840.23347700044402467Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC107020.2194897100098342No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG105880.21715165852963222TruSeq Adapter, Index 2 (97% over 35bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC99740.20455899529415866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA98220.20144159332055608Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTAGA97980.20094937195630305No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA95140.19512475247930877No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC94760.1943454019859081No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA93230.19120749078879498No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA90510.18562898199392722No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG89040.18261412613787736TruSeq Adapter, Index 2 (97% over 35bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG88930.1823885246792614No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC87670.17980436251693294No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT86730.17787649550694187TruSeq Adapter, Index 9 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC85140.17461552896876548No Hit
CGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCG84750.1738156692518543TruSeq Adapter, Index 22 (97% over 37bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC83490.17123150708952584Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA80960.1660426735413584Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG80800.1657145259651897Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC79860.16378665895519862Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC78490.1609768953342542Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC78450.16089485844021204Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG76810.1575313457844829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC68110.13968832133031026Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC67520.13847827714318822No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC63730.1307052814326923Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGA63340.12990542171578112No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG62810.12881843286972233No Hit
CGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATC61840.12682903818919963TruSeq Adapter, Index 22 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA61520.12617274303686224No Hit
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA61370.12586510468420412Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAG60950.1250037172967613No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG60870.12483964350867695No Hit
ACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTAAG59390.12180427842911654No Hit
CTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAG58730.12045066967742069TruSeq Adapter, Index 22 (97% over 37bp)
CTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGC58520.12001997598369926TruSeq Adapter, Index 22 (97% over 37bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC58040.11903553325519318Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC57550.11803058130317658Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA56900.11669748177499126No Hit
CCCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGA56870.11663595410445964No Hit
TAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAA56590.11606169584616442No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC56110.11507725311765835Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT55650.11413382883617336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG55100.11300582154309348No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG54230.11122151909767622Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCAC54140.11103693608608131No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC53950.110647260839381No Hit
ACTCTGAGTCTTAACCCAATTTTTTGAGGCCTTGCTTTGGCAAGGCTAGA53920.11058573316884936No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGAT53910.11056522394533883No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC53430.10958078121683275Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG52810.10830920935917905No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCA52380.10742731274822569TruSeq Adapter, Index 22 (97% over 37bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACA52060.1067710175958883No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGT51400.10541740884419246TruSeq Adapter, Index 9 (97% over 36bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCG51300.10521231660908703No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGA49850.10223847920005824No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC49720.1019718592944212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)