FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002433593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4490365
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2065344.599492468875025Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC881831.9638269940194173Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG857211.9089984889869756No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC755801.683159386820448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA743641.6560791828726618Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC738161.6438752751725083Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC644011.434204123718228No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTATGCCG571971.2737717312512458TruSeq Adapter, Index 6 (96% over 32bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC563821.2556217590329517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA486611.083675825907248No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC479671.0682205121409951Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTA473831.0552148878765981TruSeq Adapter, Index 6 (97% over 36bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC385840.8592619976327092TruSeq Adapter, Index 5 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC310940.6924604124609024Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT299340.6666273231686065Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG296960.6613270858827734No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA291460.6490786383734953Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC247910.5520932040045742Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC229410.5108938805642749No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG227790.5072861560251783No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC212190.4725451049079529Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG176780.3936873728527636Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG170670.3800804611651837No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC170260.37916739507812836No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC169710.37794255032720053Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG163180.36340030264800305No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC146400.3260314027924234Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC146220.3256305445103015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTC144960.3228245365354487TruSeq Adapter, Index 6 (97% over 36bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA144870.32262410739438774Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG143320.31917227218722755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC129450.28828391455928415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC129440.2882616446547218Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCAT124020.2761913563819422TruSeq Adapter, Index 6 (97% over 36bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC117290.26120371061149816TruSeq Adapter, Index 5 (97% over 35bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG113440.2526297973550034Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC108710.2420961324970242Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC98050.21835641423358682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT97230.21653028205947622Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC96030.21385789351199735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC95550.21278893809300578Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC94080.2095152621223442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG92900.20688741338398992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG89710.1997833138286086No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA89340.19895932735980262No Hit
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT88050.1960865096712628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC86740.19316915217359837Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT86740.19316915217359837Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT84930.1891382994478177Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC83730.18646591090033884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC81830.18223462903349727Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC79690.17746886945715995Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC78380.1745515119594955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG77610.17283672930819655No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA75280.16764784154517506No Hit
AACCCGTAGATCCGAACTTGCAGATCGGAAGAGCACACGTCTGAACTCCA74830.1666456958398705Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC73100.1627930023505884Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA70330.1566242387868247Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC70090.15608976107732891Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG68590.1527492753929803Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT65300.1454224767919757TruSeq Adapter, Index 6 (97% over 36bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT64800.14430898156385952Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC63860.14221561053500106TruSeq Adapter, Index 5 (97% over 35bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA63750.14197064158481548Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA62540.13927598313277428No Hit
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC61500.1369599130582926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA61150.13618046639861126Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC61070.13600230716211265No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC60820.13544555954805457Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC60480.13468838279293555No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA59940.13348580794657006No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA58430.1301230523576591Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG57390.1278069822831774Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC55640.12390974898477072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGA55580.12377612955739678No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG53570.11929987874036965No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC52680.11731785723432281No Hit
AACCCGTAGATCCGAACTTGTGCAGATCGGAAGAGCACACGTCTGAACTC52520.11696153876132563Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC52470.11685018923851401Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC50720.11295295594010732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTC50490.11244074813517387TruSeq Adapter, Index 6 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT50260.11192854033024041Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC50000.11134952281161999No Hit
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC49480.11019148777437913Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC49440.11010240815612984No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC48740.10854351483676718Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT48080.10707370113565379Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG47850.10656149333072033Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT47520.10582658648016365No Hit
CACCCGTAGAACCGACCTTGCGAGATCGGAAGAGCACACGTCTGAACTCC47160.10502486991591997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG46910.10446812230186188Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG46350.10322100764637172No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC45690.10175119394525835Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC45440.10119444633120026Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)