FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5848687
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3344995.719215270025563Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1132101.9356481206807614Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1125551.924449025909576Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1097161.8759082166646976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA1033251.7666358278362306Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTATGCCG943491.6131654848344594TruSeq Adapter, Index 18 (97% over 34bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC914211.5631029665290688No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG851141.4552667974880518No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA720391.2317123484296562No Hit
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC649071.1097704493333291Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT570710.9757916605898042TruSeq Adapter, Index 1 (97% over 35bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC450100.7695744361084804Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC428100.7319591559609875No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGTA417790.7143312678555033TruSeq Adapter, Index 18 (97% over 38bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC415520.7104500548584666Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT386330.6605414172445885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC301130.5148676959461158Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG291590.49855634264579385No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC255250.43642273898398054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG252190.43119079547255645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA240020.41038270640914787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAGTC235760.4030990203442243TruSeq Adapter, Index 18 (97% over 38bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG232180.3969779883929504No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC232020.39670442271915046Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC221720.3790936324682788Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAG215790.3689546046830682TruSeq Adapter, Index 18 (97% over 38bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC212710.3636884654624192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG187470.3205334804204773No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC183470.31369433857547857No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG178720.3055728576345426Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG171320.29292044522129496No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT165100.282285579652322TruSeq Adapter, Index 1 (97% over 35bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT160100.2737366523460736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC154240.2637173095431505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA150440.2572201247904017Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC146310.2501587108354405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG126900.21697177503258425Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC124210.2123724521418226Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT123040.21037200315216048Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG121760.2081834777617609No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT119170.20375513341712423TruSeq Adapter, Index 1 (97% over 35bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC118080.20189146726436205Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC115980.19830091779573775No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC110530.188982587031927No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG109800.18773444364521474Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA109640.18746087797141478No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC105660.18065593183564105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT105180.1798352348142412Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC103930.17769800298767913Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC103820.17750992658694165Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC101050.17277382085928003Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC99880.1707733718696179Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA99300.1697816963020931Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC96580.16513107984749398Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC94650.1618311939072821Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC94370.1613524539781322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG93060.1591126350238951No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA92630.15837742727555776No Hit
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC91940.15719767530729545Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG91230.1559837276298082Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA90310.1544107250054585No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC89270.15263254812575883Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT88770.151777655395134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC87850.1502046527707843No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC87160.14902490080252198No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG85280.1458105041353726Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT84140.14386134870954798Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT83280.14239093321287324No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC81560.1394501022195238Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC80590.13779161032211162Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC79650.1361844119885369Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGA75710.1294478572712132No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC75520.12912299803357574No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA73750.12609667776716382No Hit
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT73330.12537856787343896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC72510.12397654379521421Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC71370.12202738836938956No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC71210.12175382269558964Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA69920.11954819945057754Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC68590.11727418478711547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA68080.11640219420187813Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA67000.11455562590372848Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG66870.11433335379376602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA66120.11305101469782876No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC65840.11257227476867886Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCA65550.11207643698491646TruSeq Adapter, Index 1 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC63170.10800714758714221No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG62660.10713515700190487Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC60920.10416013029933044Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC60770.10390366248014297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCAG60660.10371558607940551TruSeq Adapter, Index 18 (97% over 38bp)
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC59560.10183482207203086No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT59390.10154415854361842No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC59080.10101412505063101No Hit
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC58830.10058667868531859Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG58530.1000737430469437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)