FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906797
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2206464.496741968334944Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1767923.603002121343108No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1215382.476931489116016Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC971301.9794990499912672Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC919471.8738700622829922Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA842581.7171690616098443Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG699791.4261645631559652TruSeq Adapter, Index 16 (97% over 49bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC683551.3930676162066618No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC603201.2293151724026896Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG567361.1562736343076756No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC519231.0581852071728257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC471830.9615845122592191Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA394380.8037422375533367TruSeq Adapter, Index 16 (97% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA343560.7001716190826724No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA317670.6474080749621393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC313660.6392357376920219TruSeq Adapter, Index 5 (97% over 35bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT311800.6354450775118677Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC281800.5743053971868003Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG280460.5715744914656139Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC245410.5001429649524934Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC232830.4745050590028485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG228910.4665161407737064Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG224390.4573044289380629No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC199100.405763678424031Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG193480.3943101783098017Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT187230.38157274490874593Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA179610.3660432661061789Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC161200.3285238822800291No Hit
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC148750.30315091494512614Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA147790.301194445174724Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC145150.29581415330611804No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC143420.2922884317407058TruSeq Adapter, Index 16 (100% over 38bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC132660.27035966639744824Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC129710.26434759783215TruSeq Adapter, Index 5 (97% over 35bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC124270.2532609357998711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC118050.24058464207914043Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA112520.22931456100588632Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC112280.2288254435632858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT109390.2229356543586376Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT107190.21845207780146603Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC105690.21539509378521263Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC103810.21156367381817506Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC103660.21125797541654973Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC103230.21038163999855714TruSeq Adapter, Index 16 (100% over 38bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG94210.1919989761141535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC92470.18845287465529958Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC91730.18694476254061457TruSeq Adapter, Index 5 (97% over 35bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC91210.18588500808164674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA90930.18531437106527945TruSeq Adapter, Index 16 (97% over 39bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC90900.1852532313849544Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA89730.18286878385227676No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC86790.17687709518042014No Hit
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC85340.17392201063137522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC84160.17151718320525589Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC79790.16261116977123774Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC78960.16091963861557754Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT77200.15733277736984025Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG76450.15580428536171356Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTC74800.15244160294383485Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG74690.15221742411597625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC74340.15150412784551714Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC72880.14852866340303053Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC72750.14826372478828856Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG72580.14791726659977986No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT71040.14477876300975973TruSeq Adapter, Index 16 (100% over 37bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC70440.14355596940325838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG70070.14280191334591588No Hit
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC68920.1404582256001216Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCC68870.14035632613291318Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG67580.13772731987893527No Hit
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC67420.13740124158386824Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTAGATCGGAAGAGCACACGTCTGAACTC67370.1372993421166598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT65570.13363096129715576Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC63410.1292289043137509Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC62440.12725205464990708No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA61620.12558090338768854No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC56530.11520753762586876No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA56420.1149833587980102Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT54620.11131497797850615Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC54260.11058130181460532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA53990.11003104469167971TruSeq Adapter, Index 16 (97% over 39bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC52840.10768735694588548Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAAGTGCCTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTC52310.10660722259347595Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGCTCAGTTCAGCAGGAACAAGATCGGAAGAGCACACGTCTGAACTCCA52160.10630152419185061Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC52110.10619962472464216Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA51620.1052010099459994No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG50940.10381517719196454No Hit
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA49730.10134921008552016Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)