FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7214802
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6088558.438970327945244Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1966572.7257435477785807No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1834342.54246755489617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC1732932.401909297025753Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTA1449842.0095353968133844TruSeq Adapter, Index 8 (97% over 36bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1326421.8384704112462131No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1240791.7197838554682443No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTATGCCG1182281.6386866888377531TruSeq Adapter, Index 8 (96% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1088521.5087316325520783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC919431.2743662265437083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC913151.26566189896826TruSeq Adapter, Index 5 (97% over 35bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG634350.8792341078798835Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC611450.8474938050967997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA565330.7835696669153223Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA537920.7455783263352203Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG535040.741586532797435No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGA490740.6801849863655303TruSeq Adapter, Index 8 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT443830.6151658770400075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC439440.6090811639737306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC410080.5683870465190868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC371080.514331509028245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC370630.5137077912879661Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC337280.46748337653618216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT321440.44552851207836336Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC281470.3901285163473648Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA244210.33848468745226823Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC236650.328006229415582Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG236630.3279785086271252Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC230920.3200642235226968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG220170.30516429972714426Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC200850.278386018077835Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG194020.2689193688198235No Hit
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTA193010.267519469002753TruSeq Adapter, Index 8 (97% over 36bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT192290.26652152061830664Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAG189530.2626960518112625TruSeq Adapter, Index 8 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC168450.23347834077775106No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC165990.23006868379755954TruSeq Adapter, Index 5 (97% over 35bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC158140.21918827432824908Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA157820.21874474171293962No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC156880.217441864655468No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC154530.2141846720117891Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC142340.19728885144734395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC140630.19491872403428395Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC139820.19379603210178187No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC139820.19379603210178187Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC133810.18546593517050086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAG130930.18147414163271564TruSeq Adapter, Index 8 (97% over 36bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC129980.18015740418101564No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT127790.17712197784499145Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA124760.17292227839377988Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC124140.17206293395161779Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC122530.16983141048084202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGAGATCGGAAGAGCACACGTCTGAACTC121280.1680988612022894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA120610.1671702147889852No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC113910.15788375065594315No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT112080.15534729851214213Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT106820.1480567311479927Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTACTTGATGACAATAAAATATCTGATAAGATCGGAAGAGCACACGTCTG99510.13792478296701696Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTAAACATCCCCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCA98490.13651102275571805Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA95740.13269941434290228No Hit
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAG95180.13192323226611072Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC94510.1309945858528065No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC93610.12974715037224863Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA90640.1256306132864076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA89380.12388420361362654No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG88350.12245658300809917Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA85310.11824302316265922Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAATGGTTCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC82670.11458387908635607Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT82180.11390471976916346Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAAGCGCTCCCCTTTAGAGGTAGATCGGAAGAGCACACGTCTGAACTCC80760.11193654378872768Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC79810.11061980633702767Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT78240.10844372444316558No Hit
AACCCGTAGATCCGAACTTGTAAGATCGGAAGAGCACACGTCTGAACTCC77350.10721014935683613Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC77320.10716856817415087Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGA75350.10443807051115195TruSeq Adapter, Index 8 (97% over 36bp)
TGTAAACATCCCCGACTGGAAAAGATCGGAAGAGCACACGTCTGAACTCC75250.10429946656886774Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG74940.10386979434778666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG74860.1037589111939593No Hit
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC74460.10320449542482246TruSeq Adapter, Index 5 (97% over 35bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA73970.10252533610762984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC72610.10064032249256459Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGATCGGAAGAGCACACGTCTGAACTCCAG72490.10047399776182354Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT72200.10007204632919933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)