FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11856855
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA203296617.145912638722493Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC4373543.68861725980456Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2979312.5127320862066713TruSeq Adapter, Index 2 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2088761.7616475869865997No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1499431.264610219151706Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1264481.066454806101618No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCT1185070.9994808910119927TruSeq Adapter, Index 22 (97% over 37bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1166140.9835154431761205No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG944380.7964843965790255Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAT925540.780594854200376TruSeq Adapter, Index 22 (97% over 37bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC887400.7484278082172718Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCTCATCTCGTA840950.7092521583505913TruSeq Adapter, Index 22 (97% over 37bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC617100.5204584183579879Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC603700.5091569391714751Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT505410.4262597459444347Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT499550.4213174572852582Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA487450.411112390258631Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC449070.37874292972293244Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG443760.37426450774678444TruSeq Adapter, Index 2 (100% over 35bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA435680.3674498844761111Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA387240.3265958806108365TruSeq Adapter, Index 22 (100% over 37bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC384080.32393075566834545Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG376010.31712456633736347No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAT372280.31397870683246104TruSeq Adapter, Index 22 (97% over 37bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC370490.31246903162769557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC334890.2824442063262138Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT328340.27691997582832883Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC321110.2708222374314268Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC292640.24681081112993283Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA285600.24087331758716793No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA278330.2347418434315002No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC276030.23280203730247184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC275310.2321947936446891No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC259630.21897037620853083Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG258890.2183462646713652TruSeq Adapter, Index 2 (100% over 35bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC254440.21459316150868Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA241600.20376398294488715Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC239710.20216996834320736Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCT233830.19721081180464803TruSeq Adapter, Index 22 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC231050.1948661765704312No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA224150.18904675818334626No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG218950.184661109543804No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC213330.17992123543722177No Hit
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA202810.17104873088184008Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC201370.16983424356627452No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC200640.16921856596880033Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA195540.16491725672617233Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC192250.1621424905676927No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT189120.1595026674442759TruSeq Adapter, Index 12 (97% over 36bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG185640.15656765643165915Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA182370.1538097581525624No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT174620.1472734548917061No Hit
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG173990.14674211669114617TruSeq Adapter, Index 2 (100% over 35bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA167820.14153837590153542TruSeq Adapter, Index 22 (100% over 37bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG167620.1413696971077069Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC158100.13334058652146796Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACCCGTAGAACCGACCTTGCGAGATCGGAAGAGCACACGTCTGAACTCC156060.13162006282441677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC154620.1304055755088512Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA154420.13023689671502267Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCT147920.12475483591559483TruSeq Adapter, Index 22 (97% over 37bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC147590.1244765159057777Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC146940.12392830982583493Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC144720.12205597521433803Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG144050.1214909012550124No Hit
CGACTCTTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTAT143620.12112824184828103TruSeq Adapter, Index 22 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGTATCTCATCTCGTATGCCG142400.12009930120592688TruSeq Adapter, Index 22 (96% over 33bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC141840.11962700058320694Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG141830.11961856664351551No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG141580.11940771815122983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT138200.11655704653552733Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA137220.11573052044576745No Hit
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA132140.11144607908252231Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAGATCGGAAGAGCACACG131690.11106655179640808No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG129670.10936289597873973Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG128230.10814840866317417Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAAAACTGAGGGGCATTTTCTAGATCGGAAGAGCACACGTCTGAACTCC123200.10390613699838616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCAGATCGGAAGAGCACA121720.1026579139240549No Hit
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA121070.10210970784411211Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC120950.10200850056781498No Hit
CGACTCTTAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT119260.10058316475996376TruSeq Adapter, Index 12 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)