FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002433651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14951303
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1373270.9184951973751051Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC758050.5070126663876721Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC726620.48599108719821943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC651270.43559414186174944No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTA637990.42671197286283347TruSeq Adapter, Index 18 (97% over 37bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT536540.3588583550209637TruSeq Adapter, Index 1 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTATGCCG511590.34217084624664484TruSeq Adapter, Index 18 (96% over 33bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA473320.3165744149523289Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG469270.3138656209428703No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC396550.2652277196174808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA382840.2560579502669433No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG350480.23441435171235578Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC345890.23134438516830272No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT327650.2191447795553337No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG310880.20792836584209418No Hit
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT291330.19485258241372005No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC286870.1918695648131805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG228180.15261546100697712Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGA221230.1479670367191408No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT217170.14525155432941197TruSeq Adapter, Index 1 (97% over 35bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACA214980.14378679905022326TruSeq Adapter, Index 18 (97% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT201940.13506515117779366TruSeq Adapter, Index 1 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG193460.12939340470860633No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACC183080.12245086598806808TruSeq Adapter, Index 18 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC166290.11122107551428795No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC159470.10665960016996512Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)