FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002433652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17621377
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1661870.9430988282016779Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC897720.5094494034149545Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC860430.4882876065814834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC806980.457955130294301No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTA707970.4017676938641061TruSeq Adapter, Index 18 (97% over 37bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT634960.3603350634856743TruSeq Adapter, Index 1 (97% over 35bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA587300.33328836900771147Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG586720.3329592233342491No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTATGCCG574920.32626281135691043TruSeq Adapter, Index 18 (96% over 33bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC491320.27882043497508735Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA479330.2720161994150627No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC432510.24544619867108No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG421750.23933997893581188Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT410730.23308621114002614No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG386230.2191826438989416No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC357520.20288993306255237Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT356960.202572137239899No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG278020.1577742760965843Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGA269070.15269521785953505No Hit
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT263040.1492732378406069TruSeq Adapter, Index 1 (97% over 35bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACA251010.14244630257896418TruSeq Adapter, Index 18 (97% over 37bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT243850.1383830559893248TruSeq Adapter, Index 1 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG236620.13428008492185373No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACC210280.11933233140633674TruSeq Adapter, Index 18 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC208680.11842434334161286No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC197110.11185845464857826Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCA185700.10538336476201605No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT177660.10082072473677853Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)