FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8449181
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1182601.3996622867944242Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC534940.6331264533213338Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTA471060.5575214923197881TruSeq Adapter, Index 7 (97% over 36bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG380120.44988975854582824TruSeq Adapter, Index 2 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC329920.3904757159303369Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA324570.3841437412691242No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC319930.37865208474052103Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC295650.34991557169860604No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA271400.3212145650566605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTATGCCG239450.2834002490892312TruSeq Adapter, Index 7 (96% over 32bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG223140.2640966029725248No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT197820.2341292013983367No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG186940.22125221367609477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCA172540.2042091416907745TruSeq Adapter, Index 7 (97% over 36bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC153840.18207681904317116No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC143870.17027685878666818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT134680.1594000649293701No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG133840.1584058857302264No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATT131060.15511562599972709TruSeq Adapter, Index 7 (97% over 36bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC128660.1522751140021737Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG128250.15178985986925833TruSeq Adapter, Index 2 (100% over 35bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG112150.1327347585523378TruSeq Adapter, Index 2 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC104050.12314803056059516No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC95320.11281566816949476Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT94930.11235408496989235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGA94740.11212921110341936No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG93310.11043673937154383No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA92550.10953724390565192Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT92150.10906382523939304Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC88740.10502793110953594No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)