FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8278432
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5329836.43821197057607Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2034942.4581225043583133Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1812992.19001617697651TruSeq Adapter, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1462501.7666388997336695No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1380621.6677312805130247No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1305801.5773518463399832No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA1121501.3547251460179899TruSeq Adapter, Index 27 (97% over 39bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC947911.145035678254046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCCG700160.8457640287436076TruSeq Adapter, Index 27 (97% over 35bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC584480.7060274216180069Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC581090.7019324432452909TruSeq Adapter, Index 27 (97% over 39bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC538370.6503284679997372TruSeq Adapter, Index 1 (97% over 38bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG489010.5907036501598371Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA398930.48189077351846343Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC346220.4182192956347289No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT342180.4133391444176868Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC339520.41012597554706015Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC332180.4012595621972857Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG329510.39803431374443854No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT316470.38228253852903543Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA312220.37714871608536493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT300140.36255658076312036TruSeq Adapter, Index 1 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC285250.344570082836943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC256590.30995000019327335No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC244250.2950437957332983No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG240860.2909488173605823No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT230500.2784343701802467Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA223540.2700269809548475TruSeq Adapter, Index 1 (97% over 37bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC206960.24999903363342235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC205750.24853740418475384TruSeq Adapter, Index 1 (97% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA204630.2471844909760689No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC204160.24661675061171004Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA203470.24578325943850235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA191890.2317951032272778No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC182450.22039197761121915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT179280.21656275004735195No Hit
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA174230.21046256102604938No Hit
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC169710.20500258986242806No Hit
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA169560.20482139612912204Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT164410.19860041128561545TruSeq Adapter, Index 1 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA150640.18196682656812305No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC148390.17924892056853278Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG147790.17852414563530872No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA147730.17845166814198632No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA144310.17432045102260912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC143880.17380102898713187Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC143290.17308833363612822No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC139230.16818402325464532Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT128890.15569373523875052No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC128480.15519847236771409Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC128480.15519847236771409Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC128330.15501727863440806TruSeq Adapter, Index 27 (97% over 39bp)
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG121300.14652533233346604Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA120340.14536569244030753Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC117880.14239411521408887Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC110120.13302035941105755No Hit
CTCGGCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC109110.13180032160679703TruSeq Adapter, Index 1 (97% over 38bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT108180.13067692046029972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA107440.12978303137599004Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG106390.1285146752428479No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC102410.12370700151912825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA101300.12236616789266373No Hit
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA101020.1220279395904925Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA97150.11735314127119725TruSeq Adapter, Index 1 (97% over 37bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT96500.11656796842687117No Hit
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC95880.11581903432920632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAA95500.11536001020483107No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC94220.11381382368061972No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG93660.11313736707627725No Hit
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG93380.11279913877410602Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT93380.11279913877410602TruSeq Adapter, Index 1 (100% over 35bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATC93230.11261794504079999No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT92960.11229179632084917TruSeq Adapter, Index 8 (97% over 36bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG92460.11168781720982911No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC91330.11032282441892377Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGACGTCGCTTTTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT85860.10371529294436435Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC83630.10102154610921489Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAGATCGGAAGAGCACACG83040.10030885075821122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)