FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7664137
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA106978913.958375222154823Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC2923583.8146238774176404Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT1466831.9138880215737273TruSeq Adapter, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1331521.7373384635478204No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC1214011.5840139600844818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA849551.1084744440241607Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG847591.1059170784655858No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC763380.9960416939310976No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA671200.8757672259773018TruSeq Adapter, Index 1 (100% over 40bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC664500.8670252110576834Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA522290.6814726824429156Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG512200.6683074689296394Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG486580.6348790476996953TruSeq Adapter, Index 1 (100% over 49bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC391100.510298811203401Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC379760.4955026247573602TruSeq Adapter, Index 1 (100% over 38bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT347920.4539584822139792TruSeq Adapter, Index 1 (100% over 35bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC334770.43680064696129517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT325180.42428782262112485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA307740.40153248826319254Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT301640.39357334035130115Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC282670.3688216951236649Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC279420.36458116549847686Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC275890.3599752979363495TruSeq Adapter, Index 1 (100% over 50bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA275740.3597795811844178TruSeq Adapter, Index 1 (100% over 37bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC253480.330735215197745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC232680.3035958255965414Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC230670.30097322112065583Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC209960.2739512615706113Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT204370.2666575506152878TruSeq Adapter, Index 1 (100% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG204270.2665270727806667No Hit
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA198030.25838525590030553Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA192190.2507653503584291No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC190180.2481427458825436No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC186110.2428322980134619Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC185740.24234953002536358Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA171600.22389996420992997No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG169540.22121212081673386No Hit
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA167970.21916361881318147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC164970.2152492837745463No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG164350.21444032119989506Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC152150.19852202537611213No Hit
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT145190.18944076808647864Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC140600.18345183547736685TruSeq Adapter, Index 1 (100% over 38bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA133460.17413571808541523Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC132000.17223074169994612No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC117380.15315488227833088No Hit
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA115520.1507279945543771Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA115030.15008865316473335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT113180.1476748132242417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC112600.1469180417834389Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC112270.14648746492918904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC110330.1439561949375383No Hit
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA107340.14005490768236528Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC104660.1365581017145179Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG103640.13522722780138194Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA103210.13466617311251092TruSeq Adapter, Index 1 (100% over 40bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT102330.13351796816784461TruSeq Adapter, Index 1 (100% over 35bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC98870.12900343508995207Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC96200.12551967690556678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATCG95550.12467157098052918No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA94600.12343203155162806No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG93090.12146181624884836Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA92280.12040494578841687No Hit
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG91390.11924369306028845Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC90490.11806939254869792Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA90430.11799110584792522Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA89070.11621660729707729TruSeq Adapter, Index 1 (100% over 40bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGGAGATC86160.1124197023096012No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG85960.11215874664035885No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA85280.1112714973649349No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG84280.10996671901872318Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA80670.1052564691888989Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA80290.10476065341733845No Hit
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC80060.10446055439770974No Hit
CTCGTACGACTCTTAGCGGTGAGATCGGAAGAGCACACGTCTGAACTCCA78910.10296005929956628Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)