FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002433725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763452
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA125857414.361623707187531Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC3011863.4368420115726086Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1640491.8719678044679198TruSeq Adapter, Index 2 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1583751.8072216291023218No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC1097221.2520408624364008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC885411.010343869059818No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG839240.9576591507547482No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA782240.8926162886497238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA728330.8310994343324982TruSeq Adapter, Index 2 (100% over 40bp)
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC669930.7644590282459469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT572540.6533270222738711Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG571920.6526195385106235Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG497670.5678926523474996TruSeq Adapter, Index 2 (100% over 49bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC452660.5165316133414093Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG444540.5072658582485532TruSeq Adapter, Index 2 (100% over 38bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC444080.5067409509403372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC352130.40181654443933734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC326630.3727184219186686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC320870.3661456695375293Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT297000.3389075446524954Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT286560.3269944309616804TruSeq Adapter, Index 2 (100% over 37bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT280540.3201249918411147Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG274540.31327837477742787TruSeq Adapter, Index 2 (100% over 35bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC262910.30000734870231505Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTC256170.29231631553410686TruSeq Adapter, Index 2 (100% over 50bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC253590.28937227019672157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC253460.28922392682700837Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTC250560.28591472857955974Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCAGATCGGAAGAGCA241130.27515412876113204No Hit
NGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA237760.27130861217702795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG236890.27031585270279335TruSeq Adapter, Index 2 (100% over 35bp)
CGCGACCTCAGATCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC227680.25980629551003415Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG221350.25258311450784465TruSeq Adapter, Index 2 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA219060.2499699889952042No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCG212200.24214202348572228No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG211400.24122914121056405No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC192390.21953677614711645No Hit
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC191550.2185782497582003No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA188940.21559997133549655Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA187570.21403666043928807Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC176230.20109655418892006No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGAT168870.19269803725746432No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG167830.19151129029975858No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT167810.19148846824287963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC165880.1892861397540604Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC164350.18754025240282024Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC162570.18550908934059318No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC147290.16807303788507086No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAG146240.1668748798989257No Hit
TGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT143730.1640107117606167TruSeq Adapter, Index 2 (100% over 37bp)
ATTTGTGGTAGTGGGGGACTGCGTTCGCGCTTTCCCCTGAGATCGGAAGA135350.1544482699283342No Hit
TCGTACGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC131900.1505114651167143Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC128430.14655183824821544No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG127770.1457987103712099Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC126880.14478312884009634Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCCAGATCGGAAGAGCACACGTCTGAACT126750.14463478547038314Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA124970.14260362240815605TruSeq Adapter, Index 2 (100% over 40bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA122860.14019589540742622Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA118340.13503811055278217Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC118320.13501528849590322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC118190.13486694512619002Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCAGATCGGAAGA116420.1328471930924024No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC114510.1306676866604621Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGCAGATCGGAAGAGCACACGTCTGAACTCCAG114090.13018842346600404Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG113590.12961787204403014TruSeq Adapter, Index 2 (100% over 35bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC112300.1281458493753375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTACGACTCTTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA108770.12411775633620176Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA107170.1222919917858853TruSeq Adapter, Index 2 (100% over 40bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC104290.11900561559531563Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGG101820.11618709157076457No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGC101640.11598169305885397No Hit
CGCGACCTCAGATCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA100780.11500034461305887Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC97330.11106353980143897Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC94090.10736636658704812Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC93350.10652195048252676No Hit
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGG93020.10614538654402399No Hit
TACCTGGTTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA91940.10491299547256035TruSeq Adapter, Index 2 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA91400.10429679993682855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)