FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002433731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002433731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438369
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1703004.952929717549222No Hit
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1402854.079986761164959Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC921642.680456925943667No Hit
AACCCGTAGATCCGAACTTGTGAGATCGGAAGAGCACACGTCTGAACTCC796362.316098126757192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTA718892.090787812477369TruSeq Adapter, Index 6 (97% over 36bp)
ATCTGGAGGTAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC593471.726021843496146Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC558461.6242003112522245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG387671.1274822452156823No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC387151.1259699002637589Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA374581.0894118694066868Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGGC354631.031390173655009No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT258330.7513155219814976Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC251780.732265792298616Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC227530.6617381671368024Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTACTTAGCAGGG222420.6468764696284779No Hit
AACCCGTAGATCCGAACTTGTGTAGATCGGAAGAGCACACGTCTGAACTC211350.6146809722865696Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG199510.5802460410735438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG191170.5559903547292335No Hit
AACCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCA186220.5415939941291932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA186020.5410123229938381No Hit
CGCGACCTCAGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC175090.5092239954466784TruSeq Adapter, Index 5 (97% over 35bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGA167960.4884874194712668TruSeq Adapter, Index 6 (97% over 36bp)
ATCTGGAGGTAAGAAGCACTTTTAGATCGGAAGAGCACACGTCTGAACTC130630.3799185020572254Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGC126830.3688667504854773No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC123590.3594436780927236No Hit
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC121820.3542958885448304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC121270.3526962929226037Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGA114410.3327449729799216No Hit
ATCGTGCATCCCTTTAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCC110580.3216059707378702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC106670.31023430004167674No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTAATCCCAGGGTCGT106650.3101761329281412No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC103520.30107297965983293No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC101360.2947909313979971No Hit
AACCCGTAGATCCGAACTTGAGATCGGAAGAGCACACGTCTGAACTCCAG100170.2913299881426339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT90120.26210101359103694Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC85870.24974050196473968TruSeq Adapter, Index 5 (97% over 35bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC85140.24761740232069332Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT82100.23877600106329486Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC80650.2345588853319699Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGG77090.22420513912264797No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC76590.22275096128426009TruSeq Adapter, Index 6 (97% over 36bp)
AGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGA70360.20463190541794668No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC68650.19965861721066006No Hit
ATCTGGAGGTAAGAAGCACTTTCTAGATCGGAAGAGCACACGTCTGAACT67970.1976809353504525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG67940.19759368468014923No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG65030.1891303696607316Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA64100.1864255988813301No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG60200.17508301174190438No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC59810.1739487530279618Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC59590.17330891477907112Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA54260.157807379021856No Hit
AAAGTGCTTCCCTTTGGACTGTAGATCGGAAGAGCACACGTCTGAACTCC53880.15670220386468117Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA52930.15393926597174415No Hit
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC50000.145417783838791Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC49830.14492336337373912Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA49030.14259667883231847Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT49000.1425094281620152Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGAT48590.14131700233453712No Hit
AACCCGTAGATCCGAACTTGTGAAGATCGGAAGAGCACACGTCTGAACTC47600.13843773021452904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG47090.13695446881937337Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGTGTGAGTCTGAAACCAATTTTTTGAGGCCTTGCGTTTCTTAGCAGGG46490.13520945541330787No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG45560.13250468463390636No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC45130.13125409169289276Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG44850.13043975210339553No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC44180.12849115379995574Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG43450.12636805415590938No Hit
TTCTCGAGGAAAGAAGCACTTTAGATCGGAAGAGCACACGTCTGAACTCC42290.12299436157084945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC41940.1219764370839779No Hit
CAACTCTGAGTCTTAAGCTAATTTTTTGAGGCCTTGTTCCGACAAGGCTA41230.11991150455346707No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC40670.11828282537447261Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA39980.11627605995749729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT39340.11441471232436076Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC39040.11354220562132802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCA38690.11252428113445649Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGGAGGTAAGAAGCACTTCAGATCGGAAGAGCACACGTCTGAACTCC37000.10760916004070534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC36770.1069402382350469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC36340.10568964529403331No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCC36160.10516614127221366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT34900.10150161311947613No Hit
CAAGGGTTTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC34510.10036735440553356TruSeq Adapter, Index 6 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT34470.1002510201784625Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)